Cloning and functional expression in Escherichia coli of the gene encoding the di- and tripeptide transport protein of Lactobacillus helveticus
| Autor: | Hajime Nakajima, Bert Poolman, Edmund R.S. Kunji, Anja Hagting, Wil N. Konings |
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| Přispěvatelé: | Enzymology, GBB Microbiology Cluster, Groningen Biomolecular Sciences and Biotechnology |
| Rok vydání: | 1997 |
| Předmět: |
DNA
Bacterial Molecular Sequence Data Biological Transport Active Gene Expression Peptide Tripeptide Biology Applied Microbiology and Biotechnology chemistry.chemical_compound Bacterial Proteins Escherichia coli Amino Acid Sequence Cloning Molecular LACTOCOCCUS-LACTIS Peptide sequence chemistry.chemical_classification Dipeptide Base Sequence Sequence Homology Amino Acid Ecology Membrane transport protein Tripeptide transport Membrane Transport Proteins Dipeptides DNA Membrane transport Molecular biology Kinetics Lactobacillus Biochemistry chemistry Genes Bacterial Peptide transport DIPEPTIDE biology.protein Carrier Proteins Oligopeptides SYSTEM Research Article Food Science Biotechnology |
| Zdroj: | Scopus-Elsevier Applied and environmental microbiology, 63(6), 2213-2217. AMER SOC MICROBIOLOGY Biochemistry, 36(22). AMER CHEMICAL SOC |
| ISSN: | 1098-5336 0099-2240 |
| Popis: | The gene encoding the di- and tripeptide transport protein (DtpT) of Lactobacillus helveticus (DtpTLH) was cloned with the aid of the inverse PCR technique and used to complement the dipeptide transport-deficient and proline-auxotrophic Escherichia coli E1772. Functional expression of the peptide transporter was shown by the uptake of prolyl-[14C] alanine in whole cells and membrane vesicles. Peptide transport via DtpT in membrane vesicles is driven by the proton motive force. The system has specificity for di- and tripeptides but not for amino acids or tetrapeptides. The dtpTLH gene consists of 1,491 bp, which translates into a 497-amino-acid polypeptide. DtpTLH shows 34% identity to the di- and tripeptide transport protein of Lactococcus lactis and is also homologous to various peptide transporters of eukaryotic origin, but the similarity between these proteins is confined mainly to the N-terminal halves. |
| Databáze: | OpenAIRE |
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