Engineering the sialic acid in organs of mice using N-propanoylmannosamine
| Autor: | Daniel Gagiannis, Werner Reutter, Reinhart Gossrau, Martin Zimmermann-Kordmann, Rüdiger Horstkorte |
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| Rok vydání: | 2006 |
| Předmět: |
Male
Glycoconjugate Cell Biophysics Biology Biochemistry Pathogenesis chemistry.chemical_compound Mice In vivo medicine Animals Muscle Skeletal Molecular Biology chemistry.chemical_classification Polysialic acid Regeneration (biology) Myocardium Cell Membrane Brain Hexosamines Flow Cytometry N-Acetylneuraminic Acid Sialic acid carbohydrates (lipids) Mice Inbred C57BL medicine.anatomical_structure chemistry Liver Organ Specificity Neural cell adhesion molecule Genetic Engineering Subcellular Fractions |
| Zdroj: | Biochimica et biophysica acta. 1770(2) |
| ISSN: | 0006-3002 |
| Popis: | Sialic acids play an important role during development, regeneration and pathogenesis. The precursor of most physiological sialic acids, such as N -acetylneuraminic acid is N -acetyl- d -mannosamine. Application of the novel N -propanoylmannosamine leads to the incorporation of the new sialic acid N -propanoylneuraminic acid into cell surface glycoconjugates. Here we analyzed the modified sialylation of several organs with N -propanoylneuraminic acid in mice. By using peracetylated N -propanoylmannosamine, we were able to replace in vivo between 1% (brain) and 68% (heart) of physiological sialic acids by N -propanoylneuraminic acid. The possibility to modify cell surfaces with engineered sialic acids in vivo offers the opportunity to target therapeutic agents to sites of high sialic acid concentration in a variety of tumors. Furthermore, we demonstrated that application of N -propanoylmannosamine leads to a decrease in the polysialylation of the neural cell adhesion molecule in vivo , which is a marker of poor prognosis for some tumors with high metastatic potential. |
| Databáze: | OpenAIRE |
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