Sequence-Tagged NotI Sites of Human Chromosome 21: Sequence Analysis and Mapping
| Autor: | Nobue Oishi, Hitoshi Ichikawa, Yoshiyuki Sakaki, Takashi Ito, Takeshi Kano, Masahira Hattori, Satoru Kuhara, Hideko Ohgusu, Misao Ohki, Atushi Toyoda |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Genetics
GC Rich Sequence Base Sequence Chromosomes Human Pair 21 Sequence analysis Molecular Sequence Data Biology Methylation Polymerase Chain Reaction Sequence-tagged site Gene mapping CpG site Organ Specificity Genetic marker Humans Deoxyribonucleases Type II Site-Specific Promoter Regions Genetic Chromosome 21 Cells Cultured GC-content Repetitive Sequences Nucleic Acid Sequence Tagged Sites |
| Zdroj: | Genomics. 17:39-44 |
| ISSN: | 0888-7543 |
| DOI: | 10.1006/geno.1993.1280 |
| Popis: | The authors isolated and analyzed 19 NotI site-containing clones specific for human chromosome 21. The overall process consisted of selective isolation of NotI site-containing clones from flow-sorted chromosome 21 libraries, selection of independent clones by their restriction patterns and nucleotide sequences, and assignment of the clones to chromosome 21. Sequence analysis showed that the regions around the NotI sites had features typical of CpG islands, such as extremely high GC content, high-frequency appearance of CpG dinucleotide sequences, and lack of methylation of these CpGs. PCR conditions for these extremely G+C-rich templates were optimized to establish these NotI sites as sequence-tagged sites. 29 refs., 1 fig., 4 tabs. |
| Databáze: | OpenAIRE |
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