Effects of sampling seasons and locations on fish environmental DNA metabarcoding in dam reservoirs
Autor: | Hidetaka Ichiyanagi, Takashi Inagawa, Izumi Katano, Masayuki K. Sakata, Hideyuki Doi, Ryo O. Gotoh, Masaki Miya, Katsuki Nakai, Jiro Okitsu, Hirotoshi Sato, Kana Hayami, Hiroki Yamanaka, Toshifumi Minamoto |
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Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
0106 biological sciences
reservoir Fauna Biodiversity 010603 evolutionary biology 01 natural sciences 03 medical and health sciences lcsh:QH540-549.5 Environmental DNA Monitoring methods Ecology Evolution Behavior and Systematics Original Research 030304 developmental biology Nature and Landscape Conservation Shore fish 0303 health sciences geography geography.geographical_feature_category Ecology Sampling (statistics) environmental DNA Fishery metabarcoding Environmental science Fish lcsh:Ecology Global biodiversity |
Zdroj: | Ecology and Evolution, Vol 10, Iss 12, Pp 5354-5367 (2020) Ecology and Evolution |
ISSN: | 2045-7758 |
Popis: | Environmental DNA (eDNA) analysis has seen rapid development in the last decade, as a novel biodiversity monitoring method. Previous studies have evaluated optimal strategies, at several experimental steps of eDNA metabarcoding, for the simultaneous detection of fish species. However, optimal sampling strategies, especially the season and the location of water sampling, have not been evaluated thoroughly. To identify optimal sampling seasons and locations, we performed sampling monthly or at two‐monthly intervals throughout the year in three dam reservoirs. Water samples were collected from 15 and nine locations in the Miharu and Okawa dam reservoirs in Fukushima Prefecture, respectively, and five locations in the Sugo dam reservoir in Hyogo Prefecture, Japan. One liter of water was filtered with glass‐fiber filters, and eDNA was extracted. By performing MiFish metabarcoding, we successfully detected a total of 21, 24, and 22 fish species in Miharu, Okawa, and Sugo reservoirs, respectively. From these results, the eDNA metabarcoding method had a similar level of performance compared to conventional long‐term data. Furthermore, it was found to be effective in evaluating entire fish communities. The number of species detected by eDNA survey peaked in May in Miharu and Okawa reservoirs, and in March and June in Sugo reservoir, which corresponds with the breeding seasons of many of fish species inhabiting the reservoirs. In addition, the number of detected species was significantly higher in shore, compared to offshore samples in the Miharu reservoir, and a similar tendency was found in the other two reservoirs. Based on these results, we can conclude that the efficiency of species detection by eDNA metabarcoding could be maximized by collecting water from shore locations during the breeding seasons of the inhabiting fish. These results will contribute in the determination of sampling seasons and locations for fish fauna survey via eDNA metabarcoding, in the future. Number of detected species by eDNA metabarcoding for (a) Miharu dam reservoir, (b) Okawa dam reservoir, and (c) Sugo dam reservoir. The number of species detected by eDNA survey peaked in May in Miharu and Okawa reservoirs, and in March and June in Sugo reservoir, which corresponds with the breeding seasons of many of the fish species inhabiting the reservoirs. |
Databáze: | OpenAIRE |
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