The F2-isoprostane 8-epiprostaglandin F2alpha increases platelet adhesion and reduces the antiadhesive and antiaggregatory effects of NO
| Autor: | Valeria Zuliani, Giuseppe Andrioli, Alessandro Lechi, Pietro Minuz, Rosamaria Tommasoli, Stefania Gaino, Maurizio Degan, Riccardo Ortolani, C. Lechi |
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| Rok vydání: | 1998 |
| Předmět: |
Adult
Blood Platelets medicine.medical_specialty P-selectin Platelet Aggregation Fibrinogen receptor Receptors Thromboxane Platelet Glycoprotein GPIIb-IIIa Complex F2-isoprostanes platelet adhesion nitric oxide Fibrinogen Dinoprost Nitric Oxide Platelet Adhesiveness Internal medicine Platelet adhesiveness medicine Humans Vasoconstrictor Agents Platelet Drug Interactions Platelet activation Cells Cultured F2-Isoprostanes Dose-Response Relationship Drug Chemistry Biphenyl Compounds P-Selectin Endocrinology Biochemistry Heptanoic Acids 15-Hydroxy-11 alpha 9 alpha-(epoxymethano)prosta-5 13-dienoic Acid Platelet aggregation inhibitor Calcium Endothelium Vascular Cardiology and Cardiovascular Medicine Platelet Aggregation Inhibitors medicine.drug |
| Zdroj: | Arteriosclerosis, thrombosis, and vascular biology. 18(8) |
| ISSN: | 1079-5642 |
| Popis: | Abstract —F 2 -isoprostanes are prostaglandin (PG) isomers produced in vivo through free radical–catalyzed peroxidation of arachidonic acid, which may affect platelet function. The current study investigated the effects of 8-epiprostaglandin F 2α (8-epi-PGF 2α ) on critical events of platelet activation. A dose-dependent increase in platelet adhesion to fibrinogen- and plasma-coated microwells by 8-epi-PGF 2α (1 to 1000 nmol/L) was observed when resting platelets (plasma from 1.3±0.2% to 5.5±0.2%, EC 50 of 48 nmol/L; fibrinogen from 3.3±0.3% to 6.4±0.2%, EC 50 of 35 nmol/L; mean±SEM, n=8, P 2α in resting platelets (from 64.8±2.1% to 83.9±1.3%; n=5, P 2α (from 48.5±3.1% to 63.1±2.0%, P 2α , 30.8±6.9%; n=14, P 2α , 30.7±5.3%; n=15, P 2α . In conclusion, F 2 -isoprostanes may participate in oxidative injury by inducing platelet activation and by reducing the antiplatelet activity of NO: increased platelet adhesiveness and expression of the fibrinogen receptor are induced by nanomolar amounts of 8-epi-PG-F 2α . Platelet secretion and aggregation can also be induced in the presence of platelet agonists. |
| Databáze: | OpenAIRE |
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