Genome-Wide Identification and Characterization of the Trehalose-6-phosphate Synthetase (TPS) Gene Family in Watermelon (Citrullus lanatus) and Their Transcriptional Responses to Salt Stress
| Autor: | Gaopeng Yuan, Junpu Liu, Guolin An, Weihua Li, Wenjing Si, Dexi Sun, Yingchun Zhu |
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| Rok vydání: | 2021 |
| Předmět: |
Transcription
Genetic QH301-705.5 Amino Acid Motifs Arabidopsis Regulatory Sequences Nucleic Acid Sodium Chloride Genes Plant Article Chromosomes Plant Catalysis Citrullus Inorganic Chemistry Gene Expression Regulation Plant Gene Regulatory Networks Biology (General) Physical and Theoretical Chemistry QD1-999 gene family watermelon trehalose-6-phosphate synthetase salt stress Molecular Biology Conserved Sequence Phylogeny Spectroscopy Plant Proteins Gene Expression Profiling Organic Chemistry General Medicine Computer Science Applications Chemistry Phenotype Glucosyltransferases Organ Specificity Multigene Family Genome Plant |
| Zdroj: | International Journal of Molecular Sciences; Volume 23; Issue 1; Pages: 276 International Journal of Molecular Sciences, Vol 23, Iss 276, p 276 (2022) International Journal of Molecular Sciences |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms23010276 |
| Popis: | With the increase in watermelon cultivation area, there is an urgent need to explore enzymatic and genetic resources for the sustainable development of watermelon, especially under salt stress. Among the various compounds known, trehalose plays an important role in regulating abiotic stress tolerances in diverse organisms, including plants. Therefore, the present study comprehensively analyzed the trehalose-6-phosphate synthase (TPS) gene family in watermelon. The study analyzed the functional classification, evolutionary characteristics, and expression patterns of the watermelon TPS genes family. Seven ClTPSs were identified and classified into two distinct classes according to gene structure and phylogeny. Evolutionary analysis suggested the role of purifying selection in the evolution of the TPS family members. Further, cis-acting elements related to plant hormones and abiotic stress were identified in the promoter region of the TPS genes. The tissue-specific expression analysis showed that ClTPS genes were widely expressed in roots, stems, leaves, flowers, and fruits, while ClTPS3 was significantly induced under salt stress. The overexpression of ClTPS3 in Arabidopsis thaliana significantly improved salt tolerance. Finally, the STRING functional protein association networks suggested that the transcription factor ClMYB and ClbHLH regulate ClTPS3. Thus, the study indicates the critical role of ClTPS3 in watermelon response to salt stress. |
| Databáze: | OpenAIRE |
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