Complementary techniques (spICP-MS, TEM, and HPLC-ICP-MS) reveal the degradation of 40 nm citrate-stabilized Au nanoparticles in rat liver after intraperitoneal injection
| Autor: | Nerea Fernández-Iglesias, Cristina Sánchez-González, Juan Llopis, Roberto Álvarez-Fernández García, Carlos López-Chaves, Jörg Bettmer, Maria Montes-Bayón |
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| Rok vydání: | 2019 |
| Předmět: |
Surface Properties
medicine.medical_treatment Intraperitoneal injection Metal Nanoparticles Nanoparticle 010501 environmental sciences 01 natural sciences Biochemistry Citric Acid Mass Spectrometry Inorganic Chemistry 03 medical and health sciences Biological specimen 0302 clinical medicine Microscopy Electron Transmission medicine Animals Particle Size Rats Wistar Chromatography High Pressure Liquid 0105 earth and related environmental sciences Chemistry Rats Liver Transmission electron microscopy Colloidal gold Molecular Medicine Degradation (geology) Particle Gold Injections Intraperitoneal 030217 neurology & neurosurgery Hplc icp ms Nuclear chemistry |
| Zdroj: | Journal of Trace Elements in Medicine and Biology. 55:1-5 |
| ISSN: | 0946-672X |
| Popis: | Background Due to the increased use of engineered nanoparticles (NPs), their tracing in environmental and biological systems is of utmost importance. Besides their accumulation within a biological specimen, little is known about their degradation and transformation into corresponding low-molecular species that might influence any toxicological impact. Analytical methods Wistar rats underwent intraperitoneal injections of 40 nm citrate-stabilized gold nanoparticles. Different liver samples were analysed for the occurrence of nanoparticles and potential degradation products by means of spICP-MS, TEM and HPLC-ICP-MS. Main findings Studies using spICP-MS revealed the presence of the originally administrated Au NPs (40 nm diameter) and some evidences of other Au-containing species due to the increased background signal. Images obtained by transmission electron microscopy (TEM) showed the predominant presence of particles of significantly smaller diameter (6 ± 2 nm). As complementary method, HPLC-ICP-MS confirmed the presence of both particle types indicating a degradation of the Au NPs accompanied by detection of low-molecular Au species. Conclusions This study underlines that degradation of gold nanoparticles to low-molecular gold species might have to be taken into account in future for studies on their toxicological behaviour and their potential use in clinical applications. |
| Databáze: | OpenAIRE |
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