Panax Notoginseng Saponins Inhibits Ventricular Remodeling after Myocardial Infarction in Rats Through Regulating ATF3/MAP2K3/p38 MAPK and NF κ B Pathway
| Autor: | Haoqiang He, Guang Chen, Yun Zhang, Zhenpeng Zhang, Yongmei Liu, Zi-Cong Xie, Ru-Feng Ma, Hongzheng Li, Chenyue Liu, Jie Wang |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Male
MAPK/ERK pathway medicine.medical_specialty MAP Kinase Kinase 3 p38 mitogen-activated protein kinases Myocardial Infarction 0211 other engineering and technologies Panax notoginseng 02 engineering and technology p38 Mitogen-Activated Protein Kinases 030226 pharmacology & pharmacy Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine Fibrosis Internal medicine 021105 building & construction medicine Animals Pharmacology (medical) Myocardial infarction Protein kinase A Ventricular remodeling ATF3 Activating Transcription Factor 3 Ventricular Remodeling business.industry Kinase NF-kappa B General Medicine Saponins medicine.disease Rats Disease Models Animal Endocrinology Complementary and alternative medicine business Biomarkers |
| Zdroj: | Chinese Journal of Integrative Medicine. 26:897-904 |
| ISSN: | 1993-0402 1672-0415 |
| DOI: | 10.1007/s11655-020-2856-6 |
| Popis: | To explore whether Panax notoginseng saponins (PNS) exhibits heart protective effect in myocardial infarction (MI) rats and to identify the potential signaling pathways involved. MI rats induced by ligating the left anterior descending (LAD) coronary artery were assigned to sham coronary artery ligation or coronary artery ligation. Totally 36 Sprague-Dawley rats were randomly divided into sham group (distilled water, n=9), MI group (distilled water, n=9), PNS group (PNS, 40 mg/kg daily, n=9) and fosinopril group (FIP, 1.2 mg/kg daily, n=9) according to a random number table. The left ventricular morphology and function were conducted by echocardiography. Histological alterations were evaluated by the stainings of HE and Masson. The serum levels of C reactive protein (CRP), tumor necrosis factor α (TNF-α), growth differentiation factor-15 (GDF-15) and the ratio of metalloproteinase-9 (MMP-9) and tissue inhibitor of MMP-9 (TIMP-1) were determined by ELISA. The levels of activating transcription factor 3 (ATF3), mitogen-activated protein kinase kinase 3 (MAP2K3), p38 mitogen-activated protein kinase (p38 MAPK), phosphorylation of p38 MAPK (p-p38 MAPK), transforming growth factor-β (TGF-β1), collagen I, nuclear factor kappa B p65 (NFκB p65), phosphorylation of NFκB p65 (p-NFκB p65), and phosphorylation of inhibitory kappa Bα (p-Iκ Bα) in hearts were measured by Western blot and immunohistochemical staining, respectively. PNS improved cardiac function and fibrosis in MI rats (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink