Unlocking biodiversity and conservation studies in high-diversity environments using environmental DNA (eDNA): A test with Guianese freshwater fishes
| Autor: | Régis Vigouroux, Sébastien Brosse, Roselyne Etienne, Kévin Cilleros, Alice Valentini, Amaia Iribar, Gaël Grenouillet, Luc Allard, Tony Dejean, Pierre Taberlet |
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| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine media_common.quotation_subject Drainage basin Fish species Biodiversity Fresh Water Biology 010603 evolutionary biology 01 natural sciences 03 medical and health sciences Genetics Animals DNA Barcoding Taxonomic Environmental DNA Ecology Evolution Behavior and Systematics media_common geography geography.geographical_feature_category Ecology Local scale Fishes DNA 030104 developmental biology Fish Guyana Metagenomics Biotechnology Diversity (politics) Global biodiversity |
| Zdroj: | Molecular ecology resources. 19(1) |
| ISSN: | 1755-0998 |
| Popis: | Determining the species compositions of local assemblages is a prerequisite to understanding how anthropogenic disturbances affect biodiversity. However, biodiversity measurements often remain incomplete due to the limited efficiency of sampling methods. This is particularly true in freshwater tropical environments that host rich fish assemblages, for which assessments are uncertain and often rely on destructive methods. Developing an efficient and nondestructive method to assess biodiversity in tropical freshwaters is highly important. In this study, we tested the efficiency of environmental DNA (eDNA) metabarcoding to assess the fish diversity of 39 Guianese sites. We compared the diversity and composition of assemblages obtained using traditional and metabarcoding methods. More than 7,000 individual fish belonging to 203 Guianese fish species were collected by traditional sampling methods, and ~17 million reads were produced by metabarcoding, among which ~8 million reads were assigned to 148 fish taxonomic units, including 132 fish species. The two methods detected a similar number of species at each site, but the species identities partially matched. The assemblage compositions from the different drainage basins were better discriminated using metabarcoding, revealing that while traditional methods provide a more complete but spatially limited inventory of fish assemblages, metabarcoding provides a more partial but spatially extensive inventory. eDNA metabarcoding can therefore be used for rapid and large-scale biodiversity assessments, while at a local scale, the two approaches are complementary and enable an understanding of realistic fish biodiversity. |
| Databáze: | OpenAIRE |
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