Characterization of the product of a nonribosomal peptide synthetase-like (NRPS-like) gene using the doxycycline dependent Tet-on system in Aspergillus terreus
| Autor: | Clay C. C. Wang, Chun-Jun Guo, Wei-Wen Sun |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Transcriptional Activation
0301 basic medicine Genes Fungal 01 natural sciences Microbiology Genome Aspergillus nidulans 03 medical and health sciences Nonribosomal peptide Genetics Aspergillus terreus Peptide Synthases skin and connective tissue diseases Gene Regulation of gene expression chemistry.chemical_classification Biological Products Aspergillus biology 010405 organic chemistry Aspergillus niger Dioxolanes biology.organism_classification 0104 chemical sciences 030104 developmental biology Biochemistry chemistry Doxycycline Multigene Family |
| Zdroj: | Fungal Genetics and Biology. 89:84-88 |
| ISSN: | 1087-1845 |
| Popis: | Genome sequencing of the fungus Aspergillus terreus uncovered a number of silent core structural biosynthetic genes encoding enzymes presumed to be involved in the production of cryptic secondary metabolites. There are five nonribosomal peptide synthetase (NRPS)-like genes with the predicted A-T-TE domain architecture within the A. terreus genome. Among the five genes, only the product of pgnA remains unknown. The Tet-on system is an inducible, tunable and metabolism-independent expression system originally developed for Aspergillus niger. Here we report the adoption of the Tet-on system as an effective gene activation tool in A. terreus. Application of this system in A. terreus allowed us to uncover the product of the cryptic NRPS-like gene, pgnA. Furthermore expression of pgnA in the heterologous Aspergillus nidulans host suggested that the pgnA gene alone is necessary for phenguignardic acid (1) biosynthesis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect