Development of microsatellite markers in Fosterella rusbyi (Bromeliaceae) using 454 pyrosequencing
| Autor: | Tina Wöhrmann, Natascha Wagner, Florian Krapp, Kurt Weising, Bruno Huettel |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Bromeliaceae
education.field_of_study Genetic diversity Population Molecular Sequence Data Temperature Plant Science Sequence Analysis DNA Biology biology.organism_classification Evolutionary biology Genetic Loci Genetic structure Botany Genotype Genetics Microsatellite Polymorphic Microsatellite Marker Pyrosequencing Fosterella education Ecology Evolution Behavior and Systematics DNA Primers Microsatellite Repeats |
| Zdroj: | American journal of botany. 99(4) |
| ISSN: | 1537-2197 |
| Popis: | PREMISE OF THE STUDY Polymorphic microsatellite markers were developed for Fosterella rusbyi (Bromeliaceae) to evaluate the population genetic structure and genetic diversity of natural populations of F. rusbyi and other Fosterella species in Bolivia. METHODS AND RESULTS 454 pyrosequencing technology was used to generate 73027 sequence reads from F. rusbyi DNA, which together contained 2796 perfect simple sequence repeats (SSRs). Primer pairs were designed for 30 loci, of which 15 were used to genotype 30 F. rusbyi plants from two geographical areas in Bolivia. All markers were polymorphic, with two to nine alleles in the overall sample. Cross-species amplification was tested in 10 additional Fosterella species. Seven loci showed consistent amplification in six or more species. CONCLUSIONS The 15 SSR markers developed for F. rusbyi are promising candidates for population genetic analyses within F. rusbyi and other species of Fosterella. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text