The reactivation of stationary phase ribosomes from Bacillus stearothermophilus
| Autor: | M. García-Patrone |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
Poly U
Ribosomal Proteins Hypertonic Solutions Biophysics Bacillus Complement factor I Biochemistry Ribosome Geobacillus stearothermophilus Peptide Initiation Factors Protein biosynthesis Bacteriophages RNA Messenger Molecular Biology Messenger RNA biology fungi Cell Cycle Cell Biology Ribosomal RNA biology.organism_classification Stationary phase Protein Biosynthesis RNA Viral Ribosomes |
| Zdroj: | Biochemical and biophysical research communications. 104(4) |
| ISSN: | 0006-291X |
| Popis: | Ribosomes from stationary phase Bacillus stearothermophilus have a diminished activity in protein synthesis due to the presence of a bound protein (association factor I, AFI). This factor inhibits the binding of fmet-tRNA to ribosomes. The inhibition of protein synthesis can be reversed in systems directed by viral mRNA by the addition of an excess of high salt ribosomal wash obtained from mid-log cells. Under this condition AFI is released from ribosomes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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