Online LC–GC Analysis of Free Sterols/Stanols and Intact Steryl/Stanyl Esters in Cereals
| Autor: | Karl-Heinz Engel, Birgit Scholz, Rebecca Esche |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
chemistry.chemical_classification
Chromatography Gas Molecular Structure Normal phase food and beverages Fatty acid Esters General Chemistry Time data Sterol Ferulic acid Automation Sterols chemistry.chemical_compound Free cholesterol chemistry lipids (amino acids peptides and proteins) Gas chromatography Food science Edible Grain General Agricultural and Biological Sciences Chromatography High Pressure Liquid |
| Zdroj: | Journal of Agricultural and Food Chemistry. 61:10932-10939 |
| ISSN: | 1520-5118 0021-8561 |
| DOI: | 10.1021/jf403046z |
| Popis: | The suitability of online liquid chromatography-gas chromatography for the analysis of free sterols/stanols, steryl/stanyl fatty acid esters, and trans-steryl/stanyl ferulic acid esters in cereals is demonstrated. The silylated lipid extracts were fractionated via liquid chromatography on a normal phase, and the fractions containing the sterol classes were transferred online to the gas chromatograph for the analysis of their individual compositions. The study provides for the first time data on free sterols/stanols and intact steryl/stanyl esters in sweet corn, popcorn, and proso millet. Sweet corn revealed the highest contents of free sterols/stanols and steryl/stanyl fatty acid esters, and popcorn, in turn, the highest amounts of trans-steryl/stanyl ferulic acid esters. The distribution patterns of the proso millet samples revealed pronounced differences from those of sweet corn and popcorn as they particularly exhibited high proportions of free cholesterol and cholesteryl fatty acid esters. Furthermore, no trans-steryl/stanyl ferulic acid esters could be detected. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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