Genetic identification and antimicrobial susceptibility of clinically isolated anaerobic bacteria: A prospective multicenter surveillance study in Japan
| Autor: | Tomoyuki Yunoki, Yasufumi Matsumura, Masaki Yamamoto, Michio Tanaka, Kyoko Hamano, Satoshi Nakano, Taro Noguchi, Miki Nagao, Satoshi Ichiyama, Naohisa Fujita, Toshiaki Komori, Yukiji Yamada, Tsunehiro Shimizu, Akihiko Hayashi, Tamotsu Ono, Osamu Fukuda, Harumi Watanabe, Takeshi Higuchi, Shunji Takakura, Kunihiko Moro, Tomomi Hasegawa, Takashi Yoshida, Masayo Shigeta, Hiromi Terada, Chiyoko Fukumoto, Yoshie Mitamura, Fusayuki Tsuboi |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male Imipenem food.ingredient 030106 microbiology Microbial Sensitivity Tests Peptoniphilus Microbiology Meropenem 03 medical and health sciences Bacteria Anaerobic Clostridium food Japan RNA Ribosomal 16S polycyclic compounds medicine Prevotella Humans Public Health Surveillance biology Bacterial Infections biochemical phenomena metabolism and nutrition bacterial infections and mycoses biology.organism_classification Anti-Bacterial Agents Metronidazole 030104 developmental biology Infectious Diseases Female Anaerobic bacteria Bacteroides medicine.drug |
| Zdroj: | Anaerobe. 48 |
| ISSN: | 1095-8274 |
| Popis: | This prospective multicenter surveillance study was designed to provide antimicrobial susceptibility profiles of clinical anaerobic bacteria with genetic species identification in Japan. In 2014, a total of 526 non-duplicate clinical anaerobic isolates were collected from 11 acute-care hospitals in the Kyoto and Shiga regions of Japan. Genetic identification was performed using 16S rRNA sequencing. Minimum inhibitory concentrations were determined in the central laboratory and were interpreted using the CLSI criteria. Genetic analysis provided species-level identification for 496 isolates (83 species in 40 genera) and genus-level identification for 21 isolates (13 genera). Among these 517 isolates, the most frequent anaerobes were Bacteroides spp. (n = 207), Prevotella spp. (n = 43), Clostridium spp. (n = 40), and Peptoniphilus spp. (n = 40). B. fragilis was the most common species (n = 107) and showed 91.6%–97.2% susceptibility to β-lactam/β-lactamase inhibitor combinations (BLBLIs; ampicillin-sulbactam, amoxicillin-clavulanate, and piperacillin-tazobactam) and carbapenems (imipenem and meropenem) as well as 100% susceptibility to metronidazole. Gram-negative anaerobes were highly susceptible to metronidazole (99.0%) followed by BLBLIs and carbapenems (>90% each). BLBLIs or carbapenems also retained activity against Gram-positive anaerobes (99.5%–100%) except Clostridioides difficile . All isolates were susceptible to combinations of metronidazole with BLBLIs or carbapenems. Thus, BLBLIs or carbapenems are first choices for empirical therapy of anaerobic infections in Japan, and these antimicrobials in combination with metronidazole should be reserved for very severe infections and targeted therapy. |
| Databáze: | OpenAIRE |
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