TYPE C PARTICLES IN CULTURE OF HUMAN GLIOBLASTOMA CELLS
| Autor: | Hidemitsu Nakacawa, Nobulro Kanai, Chikao Yutani, Masaharu Satoh, Toru Hayakawa, Hajime Kitamura, Yozo Nakata, Masaharu Hori, Yukiya Sakamoto |
|---|---|
| Rok vydání: | 1979 |
| Předmět: |
Male
Budding Adolescent Brain Neoplasms Cell Ficoll RNA-Directed DNA Polymerase Glioma General Medicine Biology medicine.disease Molecular biology Virus Pathology and Forensic Medicine chemistry.chemical_compound Retroviridae medicine.anatomical_structure chemistry Cell culture Ultrastructure medicine Humans Thymidine Cells Cultured Glioblastoma |
| Zdroj: | Pathology International. 29:643-652 |
| ISSN: | 1440-1827 1320-5463 |
| DOI: | 10.1111/j.1440-1827.1979.tb00932.x |
| Popis: | A long-term cell culture of human glioblastoma was investigated microscopically, virologically, and biochemically. Reverse transcriptase activity was detected in cultured human glioblastoma cells. 3H uridine was incorporated into particles of buoyant density at 1.07 g/ml (Ficoll) which is equal to that of Oncorna virus particles, but 3H thymidine was not incorporated at all. Furthermore, reverse transcriptase activity was also demonstrated with the particles, suggesting that the cultured human glioblastoma cells were producing type C Oncorna virus. Ultrastructural observations of cell culture of glioblastoma showed type C virus particles in cisternae and culture medium. Budding of the virus was also seen on the surface of the cell. The mean diameter of the particles was approximately 100 nm. Ca. 1.1 nm of spikes protruded from the envelope. Both types of virions were observed, i.e. the doughnut-shaped type form and the solid circular form. |
| Databáze: | OpenAIRE |
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