Short-Term Binding and Hydrolysis of Cyclic 3′:5′-Adenosine Monophosphate by Aggregating Dictyostelium Cells
| Autor: | D. Malchow, G. Gerisch |
|---|---|
| Rok vydání: | 1974 |
| Předmět: |
Adenosine monophosphate
Binding Sites Multidisciplinary biology Phosphoric Diester Hydrolases Chemotaxis Hydrolysis Cell Membrane Phosphodiesterase biology.organism_classification Dictyostelium Chromatography DEAE-Cellulose Dictyostelium discoideum Cyclic AMP receptors chemistry.chemical_compound chemistry Biochemistry Chromatography Gel Cyclic AMP Myxomycetes Binding site Receptor Biological Sciences: Cell Biology |
| Zdroj: | Proceedings of the National Academy of Sciences. 71:2423-2427 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.71.6.2423 |
| Popis: | Transient binding of cyclic AMP to aggregating cells of Dictyostelium discoideum was measured under cyclic GMP excess in order to inhibit cyclic AMP hydrolysis by cell-bound phosphodiesterase. Cyclic GMP extended the period of half-maximal cyclic AMP binding from less than 5 sec to 1-2 min. With the same time course as bound cyclic AMP was released from the cells, labeled 5′-AMP appeared in the medium. Specificity, kinetics, and developmental regulation suggest that the cyclic AMP-binding sites exposed in living cells are identical with receptor sites for the chemotactic response. The functioning of the cyclic AMP-receptor/phosphodiesterase system and its formal similarity with the synaptic acetylcholine-receptor/esterase system are discussed. |
| Databáze: | OpenAIRE |
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