Enhanced Expression of Lp-PLA 2 and Lysophosphatidylcholine in Symptomatic Carotid Atherosclerotic Plaques
| Autor: | Joseph P. McConnell, Dallit Mannheim, Fredric B. Meyer, Mario Gössl, Joerg Herrmann, Daniele Versari, Lilach O. Lerman, Amir Lerman |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Carotid Artery Diseases
Male Pathology medicine.medical_specialty medicine.medical_treatment Immunoblotting Inflammation Carotid endarterectomy medicine.disease_cause Article chemistry.chemical_compound Predictive Value of Tests medicine Humans Sirius Red Aged Endarterectomy Advanced and Specialized Nursing Endarterectomy Carotid business.industry Macrophages Lysophosphatidylcholines Immunohistochemistry Up-Regulation Lipoproteins LDL Oxidative Stress Carotid Arteries Lysophosphatidylcholine chemistry 1-Alkyl-2-acetylglycerophosphocholine Esterase Biomarker (medicine) Female Collagen Neurology (clinical) medicine.symptom Cardiology and Cardiovascular Medicine business Biomarkers Immunostaining Oxidative stress |
| Zdroj: | Stroke. 39:1448-1455 |
| ISSN: | 1524-4628 0039-2499 |
| Popis: | Background and Purpose— Circulating lipoprotein-associated phospholipase A 2 (Lp-PLA 2 ) has emerged as a novel biomarker for cardiovascular diseases. However, the correlation between the plaque expression of Lp-PLA 2 and plaque oxidative stress, inflammation, and stability as well as the clinical presentation remains poorly defined, especially for cerebrovascular disease. Therefore, this study was performed to test the hypothesis that Lp-PLA 2 expression is higher in symptomatic than in asymptomatic carotid plaques of patients undergoing carotid endarterectomy. Methods— The expression of Lp-PLA 2 in 167 carotid artery plaques was determined by immunoblotting and immunostaining. Plaque oxidative stress, inflammation, and stability were quantified by NAD(P)H oxidase p67phox and MMP-2 immunoblotting, oxidized LDL (oxLDL) immunoreactivity, macrophage and Sirius red collagen staining. Lysophosphatidylcholine 16:0 (lysoPC) concentration was measured in 55 plaques using liquid chromatography tandem mass spectrometry. Results— Lp-PLA 2 expression was significantly higher in plaques of symptomatic patients than asymptomatic patients (1.66±0.19 versus 1.14±0.10, P 2 expression was related to collagen content, which was lower in plaques from symptomatic patients than in plaques from asymptomatic patients (9.1±2.2 versus 18.5±1.7% of staining/field, P P Conclusions— Symptomatic carotid artery plaques are characterized by increased levels of Lp-PLA 2 and its product lysoPC in correlation with markers of tissue oxidative stress, inflammation, and instability. These findings strongly support a role for Lp-PLA2 in the pathophysiology and clinical presentation of cerebrovascular disease. |
| Databáze: | OpenAIRE |
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