Analytical, thermodynamical and kinetic characteristics of photoluminescence immunosensor for the determination of Ochratoxin A
| Autor: | Zuzanna Pietralik, Nelya Shpyrka, Arunas Ramanavicius, Roman Viter, Maryna Savchuk, Almira Ramanaviciene, N.F. Starodub, Igor Iatsunskyi |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Ochratoxin A
Optical fiber Materials science Photoluminescence Biomedical Engineering Biophysics Analytical chemistry Food Contamination Biosensing Techniques 02 engineering and technology 01 natural sciences Antibodies law.invention symbols.namesake chemistry.chemical_compound Limit of Detection law Electrochemistry Humans Detection limit Nanotubes Chromatography 010401 analytical chemistry Substrate (chemistry) General Medicine 021001 nanoscience & nanotechnology Ochratoxins 0104 chemical sciences Gibbs free energy chemistry symbols Nanorod Gold Zinc Oxide 0210 nano-technology Selectivity Food Analysis Biotechnology |
| Zdroj: | Biosensors and Bioelectronics. 99:237-243 |
| ISSN: | 0956-5663 |
| DOI: | 10.1016/j.bios.2017.07.056 |
| Popis: | Ochratoxin A (OTA) is one of the most widespread and dangerous food contaminants. Therefore, rapid, label-free and precise detection of low OTA concentrations requires novel sensing elements with advanced bio-analytical properties. In the present paper we report photoluminescence (PL) based immunosensor for the detection of OTA. During the development of immunosensor photoluminescent ZnO nanorods (ZnO-NRs) were deposited on glass substrate. Then the ZnO-NRs were silanized and covalently modified by Protein-A (Glass/ZnO-NRs/Protein-A). The latest structure was modified by antibodies against OTA (Anti-OTA) in order to form OTA-selective layer (Glass/ZnO-NRs/Protein-A/Anti-OTA). In order to improve immunosensors selectivity the surface of Glass/ZnO-NRs/Protein-A/Anti-OTA was additionally blocked by BSA. Formed Glass/ZnO-NRs/Protein-A/BSA&Anti-OTA structures were integrated within portable fiber optic detection system, what is important for the development of low cost and portable immunosensors. The immunosensor has been tested in a wide range of OTA concentrations from 10-4ng/ml until 20ng/ml. Interaction isotherms were derived from analytical signals of immunosensor. Association constant and Gibbs free energy for the interaction of Glass/ZnO-NRs/Protein-A/Anti-OTA with OTA were calculated, analyzed and compared with some other related results. Sensitivity range and limit of detection were determined as 0.1-1ng/ml and 10-2ng/ml, respectively. Interaction kinetics of ZnO-NRs with OTA was evaluated. Response time of the immunosensor toward OTA was in the range of 500-800s. Some insights related to the mechanism of PL-signal generation are proposed and discussed. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect