Immunostimulatory Effect of Sulfated Galactans from the Green Seaweed Caulerpa cupressoides var. flabellata
| Autor: | Diego Araujo Sabry, Arquimedes Paixão Santana-Filho, Cynthia Haynara Ferreira Silva, Dayanne Lopes Gomes, Guilherme L. Sassaki, Jefferson da Silva Barbosa, Hugo Alexandre Oliveira Rocha |
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| Rok vydání: | 2020 |
| Předmět: |
Cell Survival
inflammatory mediators Pharmaceutical Science 02 engineering and technology sulfated polysaccharides Article Proinflammatory cytokine green seaweed 03 medical and health sciences chemistry.chemical_compound Sulfation Adjuvants Immunologic Algae Drug Discovery Animals Caulerpa immunostimulation lcsh:QH301-705.5 Pharmacology Toxicology and Pharmaceutics (miscellaneous) 030304 developmental biology chemistry.chemical_classification 0303 health sciences Reactive oxygen species galactans biology Chemistry Macrophages Nuclear magnetic resonance spectroscopy Seaweed 021001 nanoscience & nanotechnology biology.organism_classification NMR Caulerpa cupressoides lcsh:Biology (General) Biochemistry Galactose Cytokines Sulfated polysaccharides 0210 nano-technology |
| Zdroj: | Marine Drugs Volume 18 Issue 5 Marine Drugs, Vol 18, Iss 234, p 234 (2020) |
| ISSN: | 1660-3397 |
| DOI: | 10.3390/md18050234 |
| Popis: | Sulfated polysaccharides (SPs) obtained from green seaweeds are structurally heterogeneous molecules with multifunctional bioactivities. In this work, two sulfated and pyruvated galactans were purified from Caulerpa cupressoides var. flabellata (named SP1 and SP2), and their immunostimulatory effect was evaluated using cultured murine macrophage cells. Both SPs equally increased the production of nitric oxide, reactive oxygen species, and the proinflammatory cytokines TNF-&alpha and IL-6. NMR spectroscopy revealed that both galactans were composed primarily of 3)-&beta d-Galp-(1&rarr 3) units. Pyruvate groups were also found, forming five-membered cyclic ketals as 4,6-O-(1'carboxy)-ethylidene-&beta d-Galp residues. Some galactoses are sulfated at C-2. In addition, only SP2 showed some galactose units sulfated at C-4, indicating that sulfation at this position is not essential for the immunomodulatory activity of these galactans. Overall, the data showed that the galactans of C. cupressoides exhibited immunostimulating activity with potential therapeutic applications, which can be used in the development of new biomedical products. |
| Databáze: | OpenAIRE |
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