Isolation of a receptor protein involved in attachment of human rhinoviruses
| Autor: | J E Tomassini, R J Colonno |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
Rhinovirus
medicine.drug_class Immunology Biology Monoclonal antibody medicine.disease_cause Microbiology Chromatography Affinity Cell membrane HeLa Virology medicine Humans Receptor Cell growth Immune Sera Cell Membrane Radioimmunoassay biology.organism_classification Molecular biology Molecular Weight medicine.anatomical_structure Solubility Polyclonal antibodies Insect Science biology.protein Chromatography Gel Receptors Virus Electrophoresis Polyacrylamide Gel Cell Division HeLa Cells Research Article |
| Zdroj: | Journal of virology. 58(2) |
| ISSN: | 0022-538X |
| Popis: | Human rhinoviruses can be classified into major and minor groups on the basis of receptor specificity. Recently, a mouse monoclonal antibody was isolated which selectively blocked the attachment of the major group of human rhinoviruses to cells. Using this monoclonal antibody, the cellular receptor for the major group of human rhinoviruses was isolated. A radioimmunoassay was developed by using the receptor antibody to specifically detect rhinovirus receptor during isolation. Solubilized receptor from detergent-treated HeLa cell membrane extracts eluted from gel filtration columns with an apparent molecular weight of 440,000. A cellular receptor protein, which had a molecular weight of 90,000 when analyzed on sodium dodecyl sulfate-polyacrylamide gels, was purified from solubilized extracts on an immunoaffinity column containing receptor antibody. Polyclonal rabbit antiserum, resulting from immunization with the isolated receptor protein, specifically blocked the attachment of the major group of human rhinoviruses and indicated that the 90-kilodalton protein plays a functional role in attachment. Prolonged exposure of HeLa cell monolayers with the receptor antibody showed no inhibition of cell growth and division. |
| Databáze: | OpenAIRE |
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