Determination of tebufelone, a new anti-inflammatory drug, in plasma and tissue using capillary gas chromatography/stable isotope dilution mass spectrometry
| Autor: | Matthew Joseph Doyle, Thomas H. Eichhold |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Detection limit
Chromatography Chemistry Coefficient of variation Anti-Inflammatory Agents Non-Steroidal In Vitro Techniques Isotope dilution Mass spectrometry Biochemistry Gas Chromatography-Mass Spectrometry Rats Standard curve Phenols Alkynes Animals Molecular Medicine Tissue Distribution Selected ion monitoring Gas chromatography Spectroscopy Electron ionization |
| Zdroj: | Biological Mass Spectrometry. 19:230-234 |
| ISSN: | 1096-9888 1052-9306 |
| Popis: | A capillary gas chromatographic/mass spectrometric method for the determination of tebufelone, a new anti-inflammatory drug, in plasma and rat paw tissue is described. Trideuteriated tubufelone was employed as an internal standard and the drug quantified by selected ion monitoring using a mass-selective detector following positive electron ionization (70 eV). Tebufelone was recovered from plasma (greater than 93%) and pulverized paw tissue (greater than 88%) by ethereal extraction. Standard curves were linear between 5 ng ml-1 and 5 micrograms ml-1 with a detection limit (S/N = 2) of 2 ng ml-1. The coefficient of variation for successive injections of standards (200 ng ml-1) or samples (10-1500 ng ml-1) was 7.3% and 8.5%, respectively. |
| Databáze: | OpenAIRE |
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