Quantum changes inHelicobacter pylorigene expression accompany host-adaptation
| Autor: | Arlaine Anne Amoyo, Mun Fai Loke, Eng Guan Chua, Jamuna Vadivelu, Yalda Khosravi, Shih-Wee Seow, Tim Perkins, Sven Pettersson, Barry J. Marshall, Chin Yen Tay, Michael J. Wise, Fanny Peters |
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| Přispěvatelé: | Lee Kong Chian School of Medicine (LKCMedicine), Singapore Centre for Environmental Life Sciences and Engineering |
| Rok vydání: | 2016 |
| Předmět: |
Lipopolysaccharides
0301 basic medicine Candidate gene Gene Expression Genome Microbiology Mice 03 medical and health sciences expression Gene expression Genetics Animals Allele Molecular Biology Gene Lewis antigen Recombination Genetic Helicobacter pylori biology General Medicine Full Papers host adaptation biology.organism_classification Adaptation Physiological 030104 developmental biology Host-Pathogen Interactions Mutation BabA Quantum Theory Host adaptation Bacterial outer membrane Genome Bacterial |
| Zdroj: | DNA Research: An International Journal for Rapid Publication of Reports on Genes and Genomes |
| ISSN: | 1756-1663 1340-2838 |
| DOI: | 10.1093/dnares/dsw046 |
| Popis: | Helicobacter pylori is a highly successful gastric pathogen. High genomic plasticity allows its adaptation to changing host environments. Complete genomes of H. pylori clinical isolate UM032 and its mice-adapted serial derivatives 298 and 299, generated using both PacBio RS and Illumina MiSeq sequencing technologies, were compared to identify novel elements responsible for host-adaptation. The acquisition of a jhp0562-like allele, which encodes for a galactosyltransferase, was identified in the mice-adapted strains. Our analysis implies a new β-1,4-galactosyltransferase role for this enzyme, essential for Ley antigen expression. Intragenomic recombination between babA and babB genes was also observed. Further, we expanded on the list of candidate genes whose expression patterns have been mediated by upstream homopolymer-length alterations to facilitate host adaption. Importantly, greater than four-fold reduction of mRNA levels was demonstrated in five genes. Among the down-regulated genes, three encode for outer membrane proteins, including BabA, BabB and HopD. As expected, a substantial reduction in BabA protein abundance was detected in mice-adapted strains 298 and 299 via Western analysis. Our results suggest that the expression of Ley antigen and reduced outer membrane protein expressions may facilitate H. pylori colonisation of mouse gastric epithelium. Published version |
| Databáze: | OpenAIRE |
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