Neuroprotective Secondary Metabolite Produced by an Endophytic Fungus, Neosartorya fischeri JS0553, Isolated from Glehnia littoralis
| Autor: | Soonok Kim, Ki Sung Kang, Ji Hoon Song, Changyeol Lee, Jong Hun Lee, Sang Hee Shim, Dahae Lee, Sunghee Bang |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Pyridones Neosartorya Glutamic Acid Naphthalenes Secondary metabolite Hippocampus 01 natural sciences Endophyte Mice medicine Extracellular Animals Phosphorylation Glehnia Protein kinase A Cell Line Transformed chemistry.chemical_classification Reactive oxygen species Cell Death Molecular Structure biology Chemistry Kinase 010401 analytical chemistry Biological activity General Chemistry biology.organism_classification 0104 chemical sciences Neuroprotective Agents Biochemistry Pyrones Calcium Mitogen-Activated Protein Kinases Reactive Oxygen Species General Agricultural and Biological Sciences Apiaceae 010606 plant biology & botany medicine.drug |
| Zdroj: | Journal of Agricultural and Food Chemistry. 67:1831-1838 |
| ISSN: | 1520-5118 0021-8561 |
| DOI: | 10.1021/acs.jafc.8b05481 |
| Popis: | Roots of Glehnia littoralis have been used to heal stroke as a traditional medicine. Even though many studies on this plant have been conducted, the secondary metabolites produced by its endophytes and their bioactivities have not been investigated thus far. Therefore, a new meroditerpenoid named sartorypyrone E (1) and eight known compounds (2-9) were isolated from extracts of cultured Neosartorya fischeri JS0553, an endophyte of G. littoralis. The isolated metabolites were identified using spectroscopic methods and chemical reaction, based on a comparison to literature data. Relative and absolute stereochemistries of compound 1 were also elucidated. To identify the protective effects of isolated compounds (1-9) in HT22 cells against glutamate-induced cytotoxicity, we assessed inhibition of cell death, intracellular reactive oxygen species (ROS) accumulation, and calcium ion (Ca2+) influx. Among the isolates, compound 8, identified as fischerin, showed significant neuroprotective activity on glutamate-mediated HT22 cell death through inhibition of ROS, Ca2+ influx, and phosphorylation of mitogen-activated protein kinase, including c-Jun N-terminal kinase, extracellular signal-regulated kinase, and p38. The results suggested that the metabolites produced by the endophyte N. fischeri JS0553 might be related to the neuroprotective activity of its host plant, G. littoralis. |
| Databáze: | OpenAIRE |
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