Dilated Minute Chambers in Laryngeal Vocal Fold Polyps: Histopathological and Ultrastructural Features
| Autor: | Cem Comunoglu, Halil Ibrahim Saygi, Nil Comunoglu, Haydar Murat Yener, Ismail Kocak, Sebnem Batur, İsmail Seçkin, Ozlem Tanriover, Sirin Bakti Demiray |
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| Rok vydání: | 2020 |
| Předmět: |
Larynx
Pathology medicine.medical_specialty Vocal Cords Stain Laryngeal Diseases Extracellular matrix Glycosaminoglycan 030507 speech-language pathology & audiology 03 medical and health sciences Speech and Hearing chemistry.chemical_compound Polyps 0302 clinical medicine Microscopy Electron Transmission Edema Hyaluronic acid otorhinolaryngologic diseases medicine Humans Hyaluronic Acid 030223 otorhinolaryngology biology Chemistry LPN and LVN Extracellular Matrix medicine.anatomical_structure Otorhinolaryngology Proteoglycan Laryngeal Mucosa Case-Control Studies biology.protein Ultrastructure Proteoglycans medicine.symptom 0305 other medical science |
| Zdroj: | Journal of Voice. 34:289-293 |
| ISSN: | 0892-1997 |
| DOI: | 10.1016/j.jvoice.2018.09.017 |
| Popis: | In Reinke's space of human vocal fold, type III collagen forms a three dimensional network and this contains numerous minute chambers in between these fibers. These compartments are occupied by glycosaminoglycans and glycoproteins. In laryngeal fold lesions, such as Reinke's edema and vocal fold polyps, proteoglycan (PG)/hyaluronic acid (HA) components of extracellular matrix increased. We investigated the size and quantity of the minute chambers within Reinke's space, filled with PG/HA with the aid of transmission electron microscopy. Eight vocal fold polyps and 10 mucosal biopsies (as control group) were all evaluated by light microscopy and electron microscopy. We detected that PG/HA in extracellular matrix had been increased in vocal fold lesions when compared with control group, by Alcian Blue-pH 2.5 stain. The mean volume of the chambers in Reinke's space of normal larynx was measured as 0.040233 µm2 whereas the mean volume of these chambers in vocal fold polyps was measured as 6.420221 µm2. The difference between the volumes of these chambers in vocal fold polyps and in control group was statistically significant (P = 0.001). Within these chambers PG/HA were found and PG/HA filling these chambers were increased in vocal fold polyps. We think proteoglycan and glycosaminoglycans, especially HA, play an important role in determining biochemical properties of vocal fold lesions. |
| Databáze: | OpenAIRE |
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