Differential gene expression profiling in whole blood during acute systemic inflammation in lipopolysaccharide-treated rats
| Autor: | Maribel Bruno, Sandra M. Ward, Stella O. Sieber, J. Todd Auman, B. Alex Merrick, Richard S. Paules, Charles J. Tucker, Rick D. Fannin |
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| Rok vydání: | 2005 |
| Předmět: |
Lipopolysaccharides
Male DNA Complementary Time Factors Microarray Physiology Enzyme-Linked Immunosorbent Assay Biology Systemic inflammation Rats Sprague-Dawley Gene expression Cell Adhesion Genetics medicine Animals Cluster Analysis Gene Oligonucleotide Array Sequence Analysis Whole blood Inflammation Principal Component Analysis Reverse Transcriptase Polymerase Chain Reaction Gene Expression Profiling Rats Muridae Oxygen Gene expression profiling Gene Expression Regulation Immunology RNA DNA microarray medicine.symptom Toxicogenomics |
| Zdroj: | Physiological Genomics. 21:92-104 |
| ISSN: | 1531-2267 1094-8341 |
| DOI: | 10.1152/physiolgenomics.00190.2004 |
| Popis: | Microarrays have been used to evaluate the expression of thousands of genes in various tissues. However, few studies have investigated the change in gene expression profiles in one of the most easily accessible tissues, whole blood. We utilized an acute inflammation model to investigate the possibility of using a cDNA microarray to measure the gene expression profile in the cells of whole blood. Blood was collected from male Sprague-Dawley rats at 2 and 6 h after treatment with 5 mg/kg (ip) LPS. Hematology showed marked neutrophilia accompanied by lymphopenia at both time points. TNF-α and IL-6 levels were markedly elevated at 2 h, indicating acute inflammation, but by 6 h the levels had declined. Total RNA was isolated from whole blood and hybridized to the National Institute of Environmental Health Sciences Rat Chip v.3.0. LPS treatment caused 226 and 180 genes to be differentially expressed at 2 and 6 h, respectively. Many of the differentially expressed genes are involved in inflammation and the acute phase response, but differential expression was also noted in genes involved in the cytoskeleton, cell adhesion, oxidative respiration, and transcription. Real-time RT-PCR confirmed the differential regulation of a representative subset of genes. Principal component analysis of gene expression discriminated between the acute inflammatory response apparent at 2 h and the observed recovery underway at 6 h. These studies indicate that, in whole blood, changes in gene expression profiles can be detected that are reflective of inflammation, despite the adaptive shifts in leukocyte populations that accompany such inflammatory processes. |
| Databáze: | OpenAIRE |
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