Cloning of the listeriolysin O gene and development of specific gene probes for Listeria monocytogenes
| Autor: | Atin R. Datta, B A Wentz, J Russell |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
DNA
Bacterial Bacterial Toxins Molecular Sequence Data Biology Molecular cloning medicine.disease_cause Applied Microbiology and Biotechnology Microbiology Hemolysin Proteins Nucleic acid thermodynamics Bacterial Proteins Listeria monocytogenes medicine Cloning Molecular Escherichia coli Heat-Shock Proteins Base Sequence Ecology Hybridization probe Listeriolysin O Nucleic Acid Hybridization Hemolysin biology.organism_classification Molecular biology Listeria DNA Probes Oligonucleotide Probes Research Article Food Science Biotechnology |
| Zdroj: | Applied and Environmental Microbiology. 56:3874-3877 |
| ISSN: | 1098-5336 0099-2240 |
| DOI: | 10.1128/aem.56.12.3874-3877.1990 |
| Popis: | A clone containing 3.1 kb of Listeria DNA was selected from a gene library of Listeria monocytogenes Scott A strain. The Escherichia coli clone produced hemolysin on sheep blood agar and in sonicated extracts but very little in the culture supernatant. This 3.1-kb DNA fragment and a 650-bp HindIII fragment located within the listeriolysin gene were used as probes in a colony hybridization assay. Both probes were specific for L. monocytogenes and did not hybridize with any other Listeria strains at high stringency. Two synthetic probes, one from the 650-bp HindIII fragment and one from the carboxy-terminal region of the protein, were also specific for L. monocytogenes. |
| Databáze: | OpenAIRE |
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