Adipogenic Differentiation Alters Properties of Vascularized Tissue-Engineered Skeletal Muscle
| Autor: | Francisca M. Acosta, Kennedy K Howland, Katerina Stojkova, Eric M. Brey, Christopher R. Rathbone, Elizabeth Hernandez |
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| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Tissue engineered
Adipogenesis business.industry Muscle Fibers Skeletal Biomedical Engineering food and beverages Skeletal muscle Adipose tissue Bioengineering Cell Differentiation Original Articles Biochemistry Cell biology Biomaterials medicine.anatomical_structure Tissue engineering Adipose Tissue Adipocytes Medicine Humans business Muscle Skeletal Microvessel |
| Zdroj: | Tissue Eng Part A |
| Popis: | Advances in the engineering of comprehensive skeletal muscle models in vitro will improve drug screening platforms and can lead to better therapeutic approaches for the treatment of skeletal muscle injuries. To this end, a vascularized tissue-engineered skeletal muscle (TE-SkM) model that includes adipocytes was developed to better emulate the intramuscular adipose tissue that is observed in skeletal muscles of patients with diseases such as diabetes. Muscle precursor cells cultured with and without microvessels derived from adipose tissue (microvascular fragments) were used to generate TE-SkM constructs, with and without a microvasculature, respectively. TE-SkM constructs were treated with adipogenic induction media to induce varying levels of adipogenesis. With a delayed addition of induction media to allow for angiogenesis, a robust microvasculature in conjunction with an increased content of adipocytes was achieved. The augmentation of vascularized TE-SkM constructs with adipocytes caused a reduction in maturation (compaction), mechanical integrity (Young's modulus), and myotube and vessel alignment. An increase in basal glucose uptake was observed in both levels of adipogenic induction, and a diminished insulin-stimulated glucose uptake was associated with the higher level of adipogenic differentiation and the greater number of adipocytes. IMPACT STATEMENT: The findings of the current study represent the effectiveness of employing a combinatorial approach involving muscle precursor cells and microvascular fragments to create a vascularized tissue-engineered skeletal muscle model with adipocytes that induce structural and metabolic changes. This model is a platform to support the discovery of mechanisms underlying the phenomena of intramuscular adipose tissue that is characteristic of the skeletal muscles of patients with diseases. |
| Databáze: | OpenAIRE |
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