Platelet volumes in patients with ischaemic heart disease, and in controls
| Autor: | J.B. Enticknap, P.G. Gooding |
|---|---|
| Rok vydání: | 1967 |
| Předmět: |
Blood Platelets
medicine.medical_specialty Chromatography Correlation coefficient Computers Chemistry Coronary Disease General Medicine Venous blood Blood Cell Count Burette Necrosis Platelet-rich plasma Coulter counter Internal medicine medicine Cardiology Humans Platelet Electronics Particle counter Whole blood |
| Zdroj: | Journal of Atherosclerosis Research. 7:711-713 |
| ISSN: | 0368-1319 |
| DOI: | 10.1016/s0368-1319(67)80049-8 |
| Popis: | The method that we have developed measures platelet volumes by the use of an electronic particle counter (Coulter Model B) in conjunction with an automatic histogram plotter (Coulter Model J). Venous blood, collected about 1 89 hours after breakfast into plastic syringes, is added to 3.8 % sodium citrate. Whole blood is diluted 1 : 500 with formalinised particle-free saline and centrifuged at 120 x g for 120 sec to remove the majority of the red cells in a modification of the method of Williamson 2. This is then further diluted I : 5 and 0.05 ml is passed through the burette of tile Coulter counter. The electrical impulses are counted and their size, which is proportional to the volume of tile cells, is recorded in 20 classes in the range of 4-17 #3. Our studies have shown that these particles are in fact blood platelets, and further confirmation is that directly comparable results have also been obtained using citrated platelet rich plasma. Ptatelet counts by visual, light microscopy methods, and from the electronic particle counter have been compared, and in a preliminary series of 50 patients studied, the correlation coefficient was 0.79, whilst in a later series when techniques had been perfected, in a series of 19 patients studied, the correlation coefficient was 0.92. The data obtained from the frequency distribution plots were handled by an I.B.M. 800 computer programmed to convert millimetre measurements of the fie |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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