Simultaneous Qualitative Assessment and Quantitative Analysis of Metabolites (Phenolics, Nucleosides and Amino Acids) from the Roots of Fresh Gastrodia elata Using UPLC-ESI-Triple Quadrupole Ion MS and ESI- Linear Ion Trap High-Resolution MS
| Autor: | Hui Xiao, Jun Zhang, Jun Qiu Liu, Sha Chen, An Liu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Glycobiology
lcsh:Medicine 01 natural sciences High-performance liquid chromatography Plant Roots Biochemistry Mass Spectrometry Analytical Chemistry chemistry.chemical_compound Mathematical and Statistical Techniques Spectrum Analysis Techniques Drug Metabolism Liquid chromatography–mass spectrometry Metabolites Medicine and Health Sciences Amino Acids lcsh:Science Chromatography High Pressure Liquid Liquid Chromatography Principal Component Analysis Multidisciplinary Gastrodia biology Chromatographic Techniques Nucleosides Glycosylamines Triple quadrupole mass spectrometer Chemistry Physical Sciences Metabolome Amino Acid Analysis Ion trap Statistics (Mathematics) Research Article Spectrometry Mass Electrospray Ionization Liquid Chromatography-Mass Spectrometry Mass spectrometry Research and Analysis Methods Phenols Chemical Analysis Metabolomics Pharmacokinetics Gastrodin Quadrupole ion trap Statistical Methods Molecular Biology Techniques Molecular Biology Pharmacology Molecular Biology Assays and Analysis Techniques Chromatography 010405 organic chemistry 010401 analytical chemistry lcsh:R Chemical Compounds Reproducibility of Results Biology and Life Sciences biology.organism_classification Gastrodia elata 0104 chemical sciences Metabolism chemistry Multivariate Analysis lcsh:Q Qualitative Analysis Mathematics |
| Zdroj: | PLoS ONE, Vol 11, Iss 3, p e0150647 (2016) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | A sensitive, effective and optimized method, based on ultra performance liquid chromatography (UPLC) coupled with ESI-triple quadrupole ion MS and ESI-linear ion trap high-resolution MS, has been developed for the simultaneous quantitative and qualitative determination of phenolics, nucleosides and amino acids in the roots of fresh Gastrodia elata. Optimization of the analytical method provided higher separation efficiency and better peak resolution for the targeted compounds. The simultaneous separation protocols were also optimized by routinely using accurate mass measurements, within 5 ppm error, for each molecular ion and the subsequent fragment ions. In total, 31 compounds, including 23 phenolics, two nucleosides, four amino acids, one gastrodin and one other compound were identified or tentatively characterized. Mono-substituted parishin glucoside (9), methoxy mono-substituted parishin (13), methyl parishin (26), p-hydroxybenzyl di-substituted parishin (29), and p-hydroxybenzyl parishin (31) were tentatively identified as new compounds. Principal metabolite content analysis and the composition of eight representative G. elata cultivars of various species indicated that geographic insulation was the main contributor to clustering. |
| Databáze: | OpenAIRE |
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