Aldose reductase inhibitory activity and antioxidant capacity of pomegranate extracts
| Autor: | Milan Stefek, Lucia Kovacikova, Ahmet Cumaoğlu, Murat Kartal, Ali Rifat Gürpinar, Ivana Milackova, Çimen Karasu |
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| Rok vydání: | 2012 |
| Předmět: |
aldose reductase inhibition
antioxidant Antioxidant DPPH Health Toxicology and Mutagenesis medicine.medical_treatment Toxicology Inhibitory postsynaptic potential Pomegranate chemistry.chemical_compound diabetic complications medicine Pharmacology chemistry.chemical_classification Aldose reductase biology Traditional medicine business.industry biology.organism_classification In vitro Antioxidant capacity Enzyme chemistry Punica Original Article business |
| Zdroj: | Interdisciplinary Toxicology |
| ISSN: | 1337-9569 1337-6853 |
| Popis: | Aldose reductase inhibitory activity and antioxidant capacity of pomegranate extractsThe pomegranate,Punica granatumL., has been the subject of current interest as a medicinal agent with wide-ranging therapeutic indications. In the present study, pomegranate ethanolic seed and hull extracts were tested, in comparison with a commercial sample, for the inhibition of aldose reductase, an enzyme involved in the etiology of diabetic complications. In vitro inhibition of rat lens aldose reductase was determined by a conventional method. Pomegranate ethanolic hull extract and commercial pomegranate hull extract exhibited similar aldose reductase inhibitory activity characterized by IC50values ranging from 3 to 33.3 μg/ml. They were more effective than pomegranate ethanolic seed extract with IC50ranging from 33.3 to 333 μg/ml. Antioxidant action of the novel compounds was documented in a DPPH test and in a liposomal membrane model, oxidatively stressed by peroxyl radicals. All the plant extracts showed considerable antioxidant potential in the DPPH assay. Pomegranate ethanolic hull extract and commercial pomegranate hull extract executed similar protective effects on peroxidatively damaged liposomal membranes characterized by 10 < IC50< 100 μg/ml. Pomegranate ethanolic seed extract showed significantly lower antioxidant activity compared to both hull extracts studied. Pomegranate extracts are thus presented as bifunctional agents combining aldose reductase inhibitory action with antioxidant activity and with potential therapeutic use in prevention of diabetic complications. |
| Databáze: | OpenAIRE |
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