Endometrial membrane organoids from human embryonic stem cell combined with the 3D Matrigel for endometrium regeneration in asherman syndrome
| Autor: | Xiangwei Fei, Meijun Guo, Xingmiao Li, Yangyang Li, Jiajie Shen, Xiuxiu Jiang, Jianhua Chen |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
3D culture
Stromal cell QH301-705.5 Asherman's syndrome 0206 medical engineering Biomedical Engineering 02 engineering and technology Biology Article Biomaterials Cell therapy medicine Progenitor cell Biology (General) Materials of engineering and construction. Mechanics of materials Mesenchymal stem cell Rat AS model 021001 nanoscience & nanotechnology medicine.disease 020601 biomedical engineering Embryonic stem cell H9-ESC Asherman Syndrome Cancer research TA401-492 0210 nano-technology Endometrial membrane organoids Biotechnology Adult stem cell |
| Zdroj: | Bioactive Materials, Vol 6, Iss 11, Pp 3935-3946 (2021) Bioactive Materials |
| Popis: | Asherman's syndrome (AS), a leading cause of uterine infertility worldwide, is characterized by scarring of the uterine surfaces lacking endometrial epithelial cells, which prevents endometrial regeneration. Current research on cell therapy for AS focuses on mesenchymal and adult stem cells from the endometrium. However, insufficient number, lack of purity, and rapid senescence of endometrial epithelial progenitor cells (EEPCs) during experimental processes restrict their use in cell therapies. In this study, we induced human embryonic stem cells-9 (H9-ESC) into EEPCs by optimizing the induction factors from the definitive endoderm. EEPCs, which act as endometrial epithelial cells, accompanied by human endometrial stromal cells provide a niche environment for the development of endometrial membrane organoids (EMOs) in an in vitro 3D culture model. To investigate the function of EMOs, we transplanted tissue-engineered constructs with EMOs into an in vivo rat AS model. The implantation of EMOs into the damaged endometrium facilitates endometrial regeneration and angiogenesis. Implanting EMOs developed from human embryonic stem cells into the endometrium might prove useful for “endometrial re-engineering” in the treatment of Asherman's syndrome. Graphical abstract Image 1 Highlights • FOXA2 and SOX17 are specific markers of endometrial epithelial progenitor cells (EEPCs). • EGF at 10 ng/mL promotes differentiation of endoderm epithelial cells into EEPCs. • Modified 3D culture promotes development of endometrial organoids. • Endometrial organoids promotes regeneration of endometrium in a rat model of AS. |
| Databáze: | OpenAIRE |
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