Insights into the Post-Translational Methylation of Arginine from Studies of Guanidinium-Water Nanodroplets
| Autor: | J. Scott McIndoe, Fraser Hof, Keri McQuinn |
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| Rok vydání: | 2008 |
| Předmět: |
Spectrometry
Mass Electrospray Ionization Arginine Tandem Hydrogen bond Chemistry Stereochemistry Electrospray ionization Organic Chemistry Water Hydrogen Bonding General Chemistry Methylation Mass spectrometry Catalysis Nanostructures Ion Models Chemical Organic chemistry Gases Solvent effects Protein Processing Post-Translational Guanidine |
| Zdroj: | Chemistry - A European Journal. 14:6483-6489 |
| ISSN: | 0947-6539 |
| DOI: | 10.1002/chem.200800101 |
| Popis: | Highly solvated methylated guanidinium ions ([Me nGuan(H2O)m](+); n=0-4 and 6; m=1->50), which model the various post-translationally methylated states of arginine, were generated in the electrospray ionization (ESI) source of an unmodified commercial tandem mass spectrometer. The dehydration processes of highly solvated [Me nGuan(H2O)21](+) ions were monitored by using energy-dependent ESI MS. These data, together with supporting calculations, provide a detailed picture of the interplay of methylation and hydration, and both the calculations and the experimental evidence suggest a specific structural basis for the observed effects. The ramifications for biochemical binding events that are controlled by the post-translational methylation of arginine to give dimethylarginines are discussed. |
| Databáze: | OpenAIRE |
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