Accuracy of some routine method used in clinical chemistry as judged by isotope dilution-mass spectrometry
| Autor: | O. Falk, A Bergman, Anders Kallner, Leif Svensson, Rolf Blomstrand, Olle Lantto, Ingemar Björkhem, E Akerlöf |
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| Rok vydání: | 1981 |
| Předmět: |
Radioisotope Dilution Technique
medicine.medical_specialty Hydrocortisone Urease Clinical Biochemistry Isotope dilution Gas Chromatography-Mass Spectrometry chemistry.chemical_compound Blood serum Reference Values Internal medicine medicine Humans Glucose oxidase Carbon Radioisotopes Creatinine Chromatography Nitrogen Isotopes biology Isotope Biochemistry (medical) Reference Standards Deuterium Endocrinology chemistry Isotope Labeling biology.protein Urea Uric acid Blood Chemical Analysis |
| Zdroj: | Clinical Chemistry. 27:733-735 |
| ISSN: | 1530-8561 0009-9147 |
| Popis: | Serum from patients was pooled, filtered, dispensed, and frozen. This pooled specimen was used for accuracy control in 64 participating laboratories in Sweden. Mean values ("state-of-the-art" values) were obtained for creatinine, cholesterol, glucose, urea, uric acid, and cortisol. These values were compared with values obtained with highly accurate reference methods based on isotope dilution-mass spectrometry. Differences were marked in the case of determination of creatinine and cortisol. Concerning the other components, the differences between the state-of-the-art value and the values obtained with the reference methods were negligible. Moreover, the glucose oxidase and the oxime methods for determination of glucose and urea were found to give significantly lower values than the hexokinase and urease methods, respectively. We conclude that methods with a higher degree of accuracy are required for routine determination of creatinine and cortisol. |
| Databáze: | OpenAIRE |
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