Osteoblast Precursors, but Not Mature Osteoblasts, Move into Developing and Fractured Bones along with Invading Blood Vessels
| Autor: | Martin K. Selig, Geert Carmeliet, Sophie Torrekens, Tatsuya Kobayashi, Sanford I. Roth, Christa Maes, Susan Mackem, Henry M. Kronenberg |
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| Rok vydání: | 2010 |
| Předmět: |
Male
musculoskeletal diseases Bone Regeneration Stromal cell Mice Transgenic DEVBIO Bone healing Biology Bone and Bones Article General Biochemistry Genetics and Molecular Biology Fractures Bone Mice 03 medical and health sciences Chondrocytes 0302 clinical medicine Cell Movement Pregnancy Bone cell medicine Animals Perichondrium Cell Lineage Bone regeneration Molecular Biology Endochondral ossification 030304 developmental biology 0303 health sciences Bone Development Osteoblasts Stem Cells Cartilage Osteoblast Cell Biology Anatomy Embryo Mammalian Cell biology medicine.anatomical_structure 030220 oncology & carcinogenesis Blood Vessels Female Pericytes Biomarkers Developmental Biology |
| Zdroj: | Developmental Cell. 19:329-344 |
| ISSN: | 1534-5807 |
| DOI: | 10.1016/j.devcel.2010.07.010 |
| Popis: | SummaryDuring endochondral bone development, the first osteoblasts differentiate in the perichondrium surrounding avascular cartilaginous rudiments; the source of trabecular osteoblasts inside the later bone is, however, unknown. Here, we generated tamoxifen-inducible transgenic mice bred to Rosa26R-LacZ reporter mice to follow the fates of stage-selective subsets of osteoblast lineage cells. Pulse-chase studies showed that osterix-expressing osteoblast precursors, labeled in the perichondrium prior to vascular invasion of the cartilage, give rise to trabecular osteoblasts, osteocytes, and stromal cells inside the developing bone. Throughout the translocation, some precursors were found to intimately associate with invading blood vessels, in pericyte-like fashion. A similar coinvasion occurs during endochondral healing of bone fractures. In contrast, perichondrial mature osteoblasts did not exhibit perivascular localization and remained in the outer cortex of developing bones. These findings reveal the specific involvement of immature osteoblast precursors in the coupled vascular and osteogenic transformation essential to endochondral bone development and repair. |
| Databáze: | OpenAIRE |
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