GISH: Resolving Interspecific and Intergeneric Hybrids

Autor:	Nathalie Piperidis
Rok vydání:	2020
Předmět:	genomic DNA Evolutionary biology Interspecific competition In situ hybridization Root tip Biology Meristem Mitosis Genome Hybrid
Zdroj:	Methods in Molecular Biology ISBN: 9781071609965 Methods in Molecular Biology ISBN: 9781627037662
Popis:	Genomic in situ hybridization (GISH) is an invaluable cytogenetic technique which enables the visualization of whole genomes in hybrids and polyploidy taxa. Total genomic DNA from one or two different species/genomes is used as a probe, labeled with a fluorochrome, and directly detected on mitotic chromosomes from root tip meristems. In sugarcane and sugarcane hybrids, we were able to characterize interspecific hybrids of two closely related species as well as intergeneric hybrids of two closely related genera.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4ce1f4b7ecaf9554b479d2e801780c3a https://doi.org/10.1007/978-1-0716-0997-2_19 Zobrazit plný text záznamu