Specific antigens of chicken thymus
| Autor: | B. O. Barger, Judith L. Pace, William L. Ragland, D. L. Dawe |
|---|---|
| Rok vydání: | 1978 |
| Předmět: |
Antiserum
medicine.diagnostic_test Globulin Cell Membrane Immunology Spleen Thymus Gland Immunoelectrophoresis Biology Immunosorbents Membrane medicine.anatomical_structure Antigen Biochemistry Organ Specificity Antigens Surface medicine biology.protein Animals Immunology and Allergy Chickens Ammonium sulfate precipitation |
| Zdroj: | European Journal of Immunology. 8:671-678 |
| ISSN: | 1521-4141 0014-2980 |
| DOI: | 10.1002/eji.1830080913 |
| Popis: | Purified plasma membranes from chicken thymus and bursa cells were prepared and solubilized with sarkosyl (sodium salt of N-methyl-N-(1-oxodecyl)-glycine). Antisera to solubilized thymus plasma membrane (TPM) were produced in rabbits and the globulin fraction obtained by ammonium sulfate precipitation. Four precipitating antigens were detected in solubilized TPM by immunoelectrophoresis. Following absorption with chicken serum and bursa plasma membrane (BPM) immunosorbents, three antigens, designated T1, T2, T3, were specific for the TPM fraction, and one antigen, T1, was found in soluble extracts of thymus tissue. Absorption with isolated plasma membrane and whole cells indicated that the T1 and T2 antigens in solubilized TPM are associated with the plasma membrane but not expressed on the surface of the cell. A common antigen, designated BT, was detected in BPM and TPM fractions and in membrane preparations of spleen. The antigens were not detected in any other tissues or cells including brain, circulating lymphocytes and erythrocytes. |
| Databáze: | OpenAIRE |
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