Lymphocyte subsets in porcine tonsillar crypt epithelium
Autor: | Dorothy M. Middleton, Mônica W. S. Salles |
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Rok vydání: | 2000 |
Předmět: |
CD4-Positive T-Lymphocytes
Pathology medicine.medical_specialty Swine T cell Palatine Tonsil Immunology Crypt CD8-Positive T-Lymphocytes Biology Palatine tonsil Immune system Tonsillar crypts medicine Animals B-Lymphocytes General Veterinary Crypt Epithelium Antibodies Monoclonal Epithelial Cells Receptors Antigen T-Cell gamma-delta Lymphocyte Subsets Epithelium medicine.anatomical_structure CD8 |
Zdroj: | Veterinary Immunology and Immunopathology. 77:133-144 |
ISSN: | 0165-2427 |
DOI: | 10.1016/s0165-2427(00)00231-2 |
Popis: | The palatine tonsils are part of the mucosa-associated lymphoid tissue (MALT), strategically located in the oropharynx at the entrance of respiratory and gastrointestinal tracts, and are recognized portals of entry and sites of multiplication and persistence of several pathogens in pigs. As the tonsillar crypt epithelium is in close contact with external environment and the underlying lymphoid tissue, the characterization of the intra-epithelial lymphocyte subpopulations is essential for the understanding of initial steps of pathogenesis of several diseases. In this work we investigated specific lymphocyte subsets in the tonsillar crypt epithelium of 10 adult healthy pigs, using monoclonal antibodies against lymphocyte markers CD3, CD4, CD8, gammadelta T cell receptor and immunoglobulin light-chain in an avidin-biotin immunoperoxidase technique. The crypt epithelium was usually extensively infiltrated by a diverse population of T cells and by B cells. The degree of infiltration of each subset was variable among animals and within individual animals. In the T cell population CD4 cells and gammadelta TCR cells predominated over CD8 cells. These data suggest that the crypt lymphoepithelium is capable of participating in both cellular and humoral immune responses and that gammadelta T cells may play an important role in the defense of this mucosa. |
Databáze: | OpenAIRE |
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