Experimentally Induced Periodontitis in Beagle Dogs Causes Rapid Increases in Osteoclastic Resorption of Alveolar Bone
| Autor: | Eiji Tsukada, Toshiaki Shibutani, Yukio Iwayama, Johan N. M. Heersche, Yoshinobu Murahashi |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Male
Pathology medicine.medical_specialty Periodontal Ligament Acid Phosphatase Alveolar Bone Loss Gingiva Osteoclasts Enzyme-Linked Immunosorbent Assay Pyridinium Compounds Giant Cells Beagle Bone resorption Dogs Osteoclast Alveolar Process medicine Animals Aggressive periodontitis Gingival sulcus Amino Acids Coloring Agents Periodontitis Dental alveolus Histocytochemistry Tartrate-Resistant Acid Phosphatase business.industry Cell Differentiation Gingival Crevicular Fluid medicine.disease Isoenzymes Cross-Linking Reagents medicine.anatomical_structure Connective Tissue Disease Progression Leukocytes Mononuclear Periodontics Female business Biomarkers Gingival margin |
| Zdroj: | Journal of Periodontology. 68:385-391 |
| ISSN: | 1943-3670 0022-3492 |
| DOI: | 10.1902/jop.1997.68.4.385 |
| Popis: | This study was undertaken to observe osteoclast differentiation related to inflammatory progression in aggressive periodontitis induced in beagle dogs by ligature of the gingival sulcus. To monitor osteoclastic activity, we used histochemical methods (staining for tartrate-resistant acid phosphatase [TRAP]) to visualize osteoclasts and their TRAP-positive precursors and biochemical methods (ELISA assay of pyridinium crosslinks) to detect bone matrix degradation products in gingival crevicular fluid (GCF), serum, and urine. For histochemical study, tissue specimens were prepared from 3 adult female beagle dogs induced with experimental periodontitis by silk ligature placement below the gingival margin of mandibular molars ligated for 3, 7, and 21 days. For biochemical study for pyridinoline measurement, the 24 mandibular molars of 4 male beagle dogs were ligated. GCF, urine, and serum were collected at day 0 and at 3, 7, 14, and 21 days after ligation. In the early inflammatory phase of ligature-induced periodontitis (day 3), TRAP+ mononuclear and TRAP+ multinucleated cells were present in the gingival connective tissue, and active bone-resorbing cells were found in excavated lacunae at the alveolar crest, but osteoclasts were not infiltrating the periodontal ligament during this early phase. During later stages of the inflammatory process (7 and 21 days), osteoclasts appeared at both the gingival and ligament side of the alveolar bone. Osteoclastic bone resorption appeared to be more severe on the bone surface at the gingival side than on the bone surface of the periodontal ligament side. Measurement of pyridinoline significantly increased in GCF and urine 3 days after ligation. The results suggested that bone at the crest of the alveolar bone is rapidly resorbed within 3 days of inducing experimental periodontitis. |
| Databáze: | OpenAIRE |
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