Dietary n-3 fatty acids significantly suppress lipogenesis in bovine muscle and adipose tissue: a functional genomics approach
| Autor: | Beate Hiller, Karin Nuernberg, Andrea Herdmann |
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| Rok vydání: | 2011 |
| Předmět: |
Male
medicine.medical_specialty Clinical chemistry Subcutaneous Fat Adipose tissue Gene Expression Biology Biochemistry Internal medicine Fatty Acids Omega-6 Gene expression Fatty Acids Omega-3 medicine Animals RNA Messenger Gene chemistry.chemical_classification Lipogenesis Muscles Organic Chemistry Fatty acid Cell Biology Animal Feed Enzyme assay Diet Endocrinology chemistry biology.protein Cattle Fatty Acid Synthases Sterol Regulatory Element Binding Protein 1 Stearoyl-CoA Desaturase Lipidology Acetyl-CoA Carboxylase |
| Zdroj: | Lipids. 46(7) |
| ISSN: | 1558-9307 |
| Popis: | Changes in fatty acid composition of longissimus muscle and subcutaneous adipose tissue of German Holstein bulls induced by a grass-silage/n-3 fatty acid based intervention diet versus a maize-silage/n-6 fatty acid based control diet were analyzed and related to shifts in lipogenic gene expression, protein expression, and enzyme activity patterns. Significantly higher amounts of n-3 fatty acids and by mean factors of 2.2–2.5 decreased n-6/n-3 fatty acid ratios in both tissues were obtained upon n-3 fatty acid intervention. In longissimus muscle, these changes of fatty acid profiles were associated with reduced SREBP1c (p = 0.02), ACC (p = 0.00), FAS (p = 0.10) and SCD (p = 0.03) gene expression, Δ6D (p = 0.03) and SCD (p = 0.03) protein expression as well as SCD enzyme activity (p = 0.03). In subcutaneous adipose tissue, significantly reduced ACC (p = 0.00) and FAS (p = 0.01) gene expression, SCD protein expression (p = 0.02) and SCD enzyme activity (p = 0.03) were detected upon n-3 fatty acid intervention, although lower degrees of correlation between gene and corresponding gene products were obtained in relation to longissimus muscle. The study elucidates tissue-specific functional genomic responses to dietary fatty acid manipulation in regard to fatty acid profile tailoring of animal tissues. |
| Databáze: | OpenAIRE |
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