Characterization of the interaction between astrocytes and encephalitogenic lymphocytes during the development of experimental autoimmune encephalitomyelitis (EAE) in mice
| Autor: | Zhongming Gao, Xiao-xia Zhan, Junwei Han, Lili Mu, Guangyou Wang, Hulun Li, Yu Liu, Boshi Sun, Jiabei Wang, Jinfeng Yang, L. L. Yang, Qingfei Kong, H. Q. Tao, Yumei Liu, Xinyue Wang |
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| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Encephalomyelitis
Autoimmune Experimental Lymphocyte Immunology Central nervous system Genes MHC Class II Myelitis Cell Communication Lymphocyte Activation Myelin oligodendrocyte glycoprotein Epitopes Interferon-gamma Mice medicine Immunology and Allergy Animals Lymphocytes biology Multiple sclerosis Interleukins Interleukin Original Articles Spinal cord medicine.disease Coculture Techniques Peptide Fragments Mice Inbred C57BL Disease Models Animal medicine.anatomical_structure Gene Expression Regulation Spinal Cord Astrocytes biology.protein Cytokines Female Myelin-Oligodendrocyte Glycoprotein Astrocyte |
| Popis: | Summary The nature of pathogenic mechanisms associated with the development of multiple sclerosis (MS) have long been debated. However, limited research was conducted to define the interplay between infiltrating lymphocytes and resident cells of the central nervous system (CNS). Data presented in this report describe a novel role for astrocyte-mediated alterations to myelin oligodendrocyte glycoprotein (MOG)35–55-specific lymphocyte responses, elicited during the development of experimental autoimmune encephalitomyelitis (EAE). In-vitro studies demonstrated that astrocytes inhibited the proliferation and interferon (IFN)-γ, interleukin (IL)-4, IL-17 and transforming growth factor (TGF)-β secretion levels of MOG35–55-specific lymphocytes, an effect that could be ameliorated by astrocyte IL-27 neutralization. However, when astrocytes were pretreated with IFN-γ, they could promote the proliferation and secretion levels of MOG35–55-specific lymphocytes, coinciding with apparent expression of major histocompatibility complex (MHC)-II on astrocytes themselves. Quantitative polymerase chain reaction (qPCR) demonstrated that production of IL-27 in the spinal cord was at its highest during the initial phases. Conversely, production of IFN-γ in the spinal cord was highest during the peak phase. Quantitative analysis of MHC-II expression in the spinal cord showed that there was a positive correlation between MHC-II expression and IFN-γ production. In addition, astrocyte MHC-II expression levels correlated positively with IFN-γ production in the spinal cord. These findings suggested that astrocytes might function as both inhibitors and promoters of EAE. Astrocytes prevented MOG35–55-specific lymphocyte function by secreting IL-27 during the initial phases of EAE. Then, in the presence of higher IFN-γ levels in the spinal cord, astrocytes were converted into antigen-presenting cells. This conversion might promote the progression of pathological damage and result in a peak of EAE severity. |
| Databáze: | OpenAIRE |
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