Simultaneous and rapid detection of multiple pesticide and veterinary drug residues by suspension array technology
| Autor: | Hongwei Ma, Guorong Ou, Zhixian Gao, Xiaoli Li, Ma Xinhua, Nan Liu, Pu Su |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Biomedical Engineering
Biophysics Biosensing Techniques Complex Mixtures Tylosin Sensitivity and Specificity chemistry.chemical_compound Suspension array technology Suspensions Carbaryl Electrochemistry medicine Veterinary drug Pesticides Immunoassay Detection limit Residue (complex analysis) Chromatography Reproducibility of Results Veterinary Drugs Equipment Design General Medicine Equipment Failure Analysis chemistry Clenbuterol Quantitative analysis (chemistry) Biotechnology medicine.drug |
| Zdroj: | Biosensors and Bioelectronics. 41:710-716 |
| ISSN: | 0956-5663 |
| DOI: | 10.1016/j.bios.2012.09.050 |
| Popis: | Suspension array technology is proposed for the simultaneous quantitative determination of seven kinds of pesticide and veterinary drug residues, namely, atrazine, chloramphenicol, carbaryl, clenbuterol, 17-β-estradiol, imidacloprid, and tylosin. The assay is simple and can be accomplished within 2h without repeated pumping and washing steps unlike conventional suspension arrays. The hapten-protein conjugate-coated beads bind to their complementary biotinylated antibodies using a competitive immunoassay format. The coefficients of determination R(2) for six targets were greater than 0.992, whereas that for atrazine was 0.961, which indicate good logistic correlations. The dynamic ranges for the seven targets in the 7-plex assay ranged from 2 log units to 4 log units(1.60×10(0)-1.64×10(3), 5.12×10(-2)-1.60×10(2), 1.00×10(0)-3.13×10(3), 4.00×10(-1)-4.10×10(2), 4.00×10(-1)-4.10×10(2), 5.12×10(-2)-1.60×10(2), and 2.00×10(0)-4.00×10(2)ngmL(-1)). The minimum detection concentrations of chloramphenicol, carbaryl, clenbuterol and 17-β-estradiol in the suspension array (0.05, 1.00, 0.40 and 0.40 ng mL(-1)) were lower than the corresponding limits of detection (0.25, 6.60, 24.23 and 13.96 ng mL(-1)) of using an indirect competitive enzyme-linked immunosorbent assay. Environmental scanning electron microscope was employed to characterize the bead surface, which directly confirmed the reactions on the beads. The suspension array is more flexible and feasible than ELISA for the fast quantitative analysis of pesticide and veterinary drug residues. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect