Multicolour FISH painting for the analysis of chromosomal aberrations induced by 220kV X-rays and fission neutrons
| Autor: | S, Knehr, R, Huber, H, Braselmann, H, Schraube, M, Bauchinger |
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| Rok vydání: | 1999 |
| Předmět: |
Chromosome Aberrations
Neutrons Genetics Radiological and Ultrasound Technology business.industry X-Rays Chromosome Linear energy transfer Dose-Response Relationship Radiation Radiation Biology Chromosome Painting Ionizing radiation Optics Linear term Chromosomes Human Humans Female Radiology Nuclear Medicine and imaging Lymphocytes Irradiation Fission neutron business Cells Cultured Multicolour fish |
| Zdroj: | International Journal of Radiation Biology. 75:407-418 |
| ISSN: | 1362-3095 0955-3002 |
| DOI: | 10.1080/095530099140320 |
| Popis: | The quantification of radiation-induced chromosome aberrations identified by multicoloured FISH painting and classified according to different nomenclature systems (PAINT, SS and a conventional method).Blood samples were irradiated with five different doses of 220 kV X-rays or fission neutrons respectively. Cell cycle-controlled, multicoloured FISH painting was performed with a cocktail of chromosomes 1, 4, 12 and a pancentromeric probe.Ten aberration parameters or categories were selected according to the three nomenclature systems and dose-response curves were constructed for the observed yields. Fitted coefficients of the linear-quadratic dose-response function show the relative importance of the quadratic term for aberration parameters of the low-LET radiation (X-rays) data set and of the linear term for those of the high-LET radiation (fission neutrons) data set. The relative proportion of complex aberrations observed was larger for the densely ionizing fission neutrons than for sparsely ionizing X-rays.Compared with single-colour FISH painting, a multicolour approach provides extended information for a mechanistic and quantitative interpretation of radiation-induced chromosome aberrations. The choice of a nomenclature system and the selection of an appropriate aberration parameter or category depend on these specific aspects. Practical application requires a rapid and reproducible description of the observed painting patterns and should also throw light on the origin of aberrations. |
| Databáze: | OpenAIRE |
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