Drought stress and re-watering affect the abundance of TIP aquaporin transcripts in barley

Autor: Marzena Kurowska, Klaudia Wiecha, Iwona Szarejko, Katarzyna Gajek
Rok vydání: 2019
Předmět:
0106 biological sciences
0301 basic medicine
Leaves
Cell Membranes
drought
Plant Science
01 natural sciences
Database and Informatics Methods
Plant Growth Regulators
Gene Expression Regulation
Plant
Plant Resistance to Abiotic Stress
Natural Resources
Gene expression
plant aquaporins
Promoter Regions
Genetic
Water content
Regulation of gene expression
Multidisciplinary
Ecology
Plant Anatomy
food and beverages
Eukaryota
Plants
Droughts
Horticulture
Plant Physiology
aquaporin transcripts
Water Resources
Medicine
Cellular Structures and Organelles
Sequence Analysis
Research Article
Drought Adaptation
Bioinformatics
Science
Aquaporin
Biology
Aquaporins
Research and Analysis Methods
03 medical and health sciences
Stress
Physiological
Sequence Motif Analysis
Plant-Environment Interactions
Barley
Plant Defenses
Hormone transport
Amino Acid Sequence
RNA
Messenger
Grasses
Gene
Water transport
Plant Ecology
Ecology and Environmental Sciences
Organisms
Water
Biology and Life Sciences
Membrane Proteins
Promoter
Hordeum
Cell Biology
Plant Pathology
030104 developmental biology
Seedlings
010606 plant biology & botany
Tonoplast Intrinsic Proteins
Zdroj: PLoS ONE
PLoS ONE, Vol 14, Iss 12, p e0226423 (2019)
ISSN: 1932-6203
Popis: Tonoplast Intrinsic Proteins (TIP) are plant aquaporins that are primarily localized in the tonoplast and play a role in the bidirectional flux of water and other substrates across a membrane. In barley, eleven members of the HvTIP gene subfamily have been identified. Here, we describe the transcription profile of the HvTIP genes in the leaves of barley seedlings being grown under optimal moisture conditions, drought stress and a re-watering phase. The applied drought stress caused a 55% decrease in the relative water content (RWC) in seedlings, while re-watering increased the RWC to 90% of the control. Our analysis showed that all HvTIP genes, except HvTIP3;2, HvTIP4;3 and HvTIP5.1, were expressed in leaves of ten-day-old barley seedlings under optimal water conditions with the transcripts of HvTIP2;3, HvTIP1;2 and HvTIP1;1 being the most abundant. We showed, for the first time in barley, a significant variation in the transcriptional activity between the analysed genes under drought stress. After drought treatment, five HvTIP genes, which are engaged in water transport, were down-regulated to varying degrees, while two, HvTIP3;1 and HvTIP4;1, were up-regulated. The HvTIP3;1 isoform, which is postulated as transporting hydrogen peroxide, expressed the highest increase of activity (ca. 5000x) under drought stress, thus indicating its importance in the response to this stress. Re-hydration caused the return of the expression of many genes to the level that was observed under optimal moisture conditions or, at least, a change in this direction Additionally, we examined the promotor regions of HvTIP and detected the presence of the cis-regulatory elements that are connected with the hormone and stress responses in all of the genes. Overall, our results suggest that 7 of 11 studied HvTIP (HvTIP1;1, HvTIP1;2, HvTIP2;1, HvTIP2;2, HvTIP2;3, HvTIP3;1, HvTIP4;1) have an important function during the adaptation of barley to drought stress conditions. We discuss the identified drought-responsive HvTIP in terms of their function in the adaptation of barley to this stress.
Databáze: OpenAIRE
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