MicroRNA profiling of diabetic atherosclerosis in a rat model
| Autor: | Shang Lei, Ping Sun, Jinyuan Li, Jian Zhang, Yiming Ouyang, Yuejin Li, Quan Zhao, Linhai Li, Kunmei Gong, Le Xiao |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male Rat model bcl-X Protein lcsh:Medicine Disease Computational biology 030204 cardiovascular system & hematology Carbohydrate metabolism Biology Diabetes Mellitus Experimental Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine microRNA Animals Humans Gene Regulatory Networks Gene miRNA-gene regulatory network Oligonucleotide Array Sequence Analysis Iliac artery Research Gene Expression Profiling lcsh:R Diagnostic marker General Medicine Atherosclerosis Phosphoproteins Disease Models Animal MicroRNAs 030104 developmental biology Gene Ontology Differentially expressed miRNA Diabetic atherosclerosis Microrna profiling |
| Zdroj: | European Journal of Medical Research European Journal of Medical Research, Vol 23, Iss 1, Pp 1-10 (2018) |
| ISSN: | 2047-783X 0949-2321 |
| Popis: | Objective The incidence of diabetic atherosclerosis (DA) is increasing worldwide. The study aim was to identify differentially expressed microRNAs (DE-miRs) potentially associated with the initiation and/or progression of DA, thereby yielding new insights into this disease. Methods Matched iliac artery tissue samples were isolated from 6 male rats with or without DA. The Affymetrix GeneChip microRNA 4.0 Array was used to detect miRs. Differential expression between atherosclerotic group and non-atherosclerotic group samples was analyzed using the Gene-Cloud of Biotechnology Information platform. Targetscan and miRanda were then used to predict targets of DE-miRs. Functions and pathways were identified for significantly enriched candidate target genes and a DE-miR functional regulatory network was assembled to identify DA-associated core target genes. Results A total of nine DE-miRs (rno-miR-206-3p, rno-miR-133a-5p, rno-miR-133b-3p, rno-miR-133a-3p, rno-miR-325-5p, rno-miR-675-3p, rno-miR-411-5p, rno-miR-329-3p, and rno-miR-126a-3p) were identified, all of which were up-regulated and together predicted to target 3349 genes. The target genes were enriched in known functions and pathways related to lipid and glucose metabolism. The functional regulatory network indicated a modulatory pattern of these metabolic functions with DE-miRs. The miR-gene network suggested arpp19 and MDM4 as possible DA-related core target genes. Conclusion The present study identified DE-miRs and miRNA-gene networks enriched for lipid and glucose metabolic functions and pathways, and arpp19 and MDM4 as potential DA-related core target genes, suggesting DE-miRs and/or arpp19 and MDM4 could act as potential diagnostic markers or therapeutic targets for DA. Electronic supplementary material The online version of this article (10.1186/s40001-018-0354-5) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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