Nucleotide sequence of the lecithinase operon of Listeria monocytogenes and possible role of lecithinase in cell-to-cell spread
| Autor: | Shaynoor Dramsi, Hélène Ohayon, J Mengaud, C Geoffroy, José A. Vázquez-Boland, Christine Kocks, Pascale Cossart |
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| Přispěvatelé: | Génie microbiologique, Institut Pasteur [Paris] (IP), Génétique moléculaire des listéria, Station centrale de microscopie électronique (SCME), Antigènes bactériens, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris], Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 1992 |
| Předmět: |
Operon
[SDV]Life Sciences [q-bio] Restriction Mapping Viral Plaque Assay MESH: Amino Acid Sequence Bacterial Outer Membrane Proteins/genetics MESH: Virulence MESH: Base Sequence medicine.disease_cause MESH: Listeria monocytogenes Mice MESH: Blotting Southern MESH: Animals Cloning Molecular Promoter Regions Genetic Peptide sequence MESH: Restriction Mapping 0303 health sciences Virulence Listeria monocytogenes/enzymology Nucleic acid sequence Blotting Southern MESH: Phospholipases Phospholipases/physiology Infectious Diseases MESH: Viral Plaque Assay MESH: DNA Transposable Elements Phospholipases Operon/genetics Lecithinase Bacterial Outer Membrane Proteins Research Article Phospholipases/genetics MESH: Operon Molecular Sequence Data Immunology MESH: Microscopy Electron In Vitro Techniques Molecular cloning Biology Microbiology MESH: Sequence Homology Nucleic Acid Virulence/genetics 03 medical and health sciences Sequence Homology Nucleic Acid MESH: Promoter Regions Genetic medicine Animals MESH: Cloning Molecular Amino Acid Sequence Escherichia coli MESH: Mice 030304 developmental biology Southern blot MESH: In Vitro Techniques MESH: Molecular Sequence Data Base Sequence 030306 microbiology MESH: Bacterial Outer Membrane Proteins Promoter Regions Genetic/genetics Listeria monocytogenes Molecular biology Microscopy Electron Open reading frame Listeria monocytogenes/pathogenicity DNA Transposable Elements Parasitology |
| Zdroj: | Infection and Immunity Infection and Immunity, 1992, 60 (1), pp.219-230. ⟨10.1128/iai.60.1.219-230.1992⟩ Infection and Immunity, American Society for Microbiology, 1992, 60 (1), pp.219-230. ⟨10.1128/iai.60.1.219-230.1992⟩ Vazquez-Boland, J A, Kocks, C, Dramsi, S, Ohayon, H, Geoffroy, C, Mengaud, J & Cossart, P 1992, ' Nucleotide sequence of the lecithinase operon of Listeria monocytogenes and possible role of lecithinase in cell-to-cell spread ', Infection and Immunity, vol. 60, no. 1, pp. 219-30 . |
| ISSN: | 0019-9567 1098-5522 |
| DOI: | 10.1128/iai.60.1.219-230.1992⟩ |
| Popis: | International audience; The lecithinase gene of the intracellular pathogen Listeria monocytogenes, plcB, was identified in a 5,648-bp DNA fragment which expressed lecithinase activity when cloned into Escherichia coli. This fragment is located immediately downstream of the previously identified gene mpl (prtA). It contains five open reading frames, named actA, plcB, and ORFX, -Y, and -Z, which, together with mpl, form an operon, since a 5.7-kb-long transcript originates from a promoter located upstream of mpl (J. Mengaud, C. Geoffroy, and P. Cossart, Infect. Immun. 59:1043-1049, 1991). A second promoter was detected in front of actA which encodes a putative membrane protein containing a region of internal repeats. plcB encodes the lecithinase, a predicted 289-amino-acid protein homologous to the phosphatidylcholine-specific phospholipases C of Bacillus cereus and Clostridium perfringens (alpha-toxin). plcB mutants produce only small plaques on fibroblast monolayers, and an electron microscopic analysis of infected macrophages suggests that lecithinase is involved in the lysis of the two-membrane vacuoles that surround the bacteria after cell-to-cell spread. On the opposite DNA strand, downstream of the operon, three more open reading frames, ldh, ORFA, and ORFB, were found. The deduced amino acid sequence of the first one is homologous to lactate dehydrogenases. Low-stringency Southern hybridization experiments suggest that these three open reading frames lie outside of the L. monocytogenes virulence region: mpl and actA were specific for L. monocytogenes, sequences hybridizing to plcB were detected in L. ivanovii and L. seeligeri, and sequences hybridizing to ORFX, -Y, and -Z were found in L. innocua. In contrast to this, sequences hybridizing to ldh or ORFB were detected in all Listeria species (including the nonpathogenic ones). |
| Databáze: | OpenAIRE |
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