Characterization of a myxoma virus-encoded serpin-like protein with activity against interleukin-1 beta-converting enzyme

Autor: F. Petit, Stéphane Bertagnoli, C. Boucraut-Baralon, F Fassy, Alain Milon, Jacqueline Gelfi
Přispěvatelé: ProdInra, Migration, Interactions hôtes-agents pathogènes [Toulouse] (IHAP), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées
Rok vydání: 1996
Předmět:
Transcription
Genetic
Immunology
Molecular Sequence Data
Myxoma virus
Biology
Serpin
Kidney
Microbiology
Cell Line
Substrate Specificity
03 medical and health sciences
Open Reading Frames
Viral Proteins
Virology
medicine
Animals
Humans
Genomic library
Amino Acid Sequence
Peptide sequence
Gene
ComputingMilieux_MISCELLANEOUS
Serpins
030304 developmental biology
[SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology
0303 health sciences
BIOTECHNOLOGIE
Genomic Library
Myxomatosis
Base Sequence
Sequence Homology
Amino Acid
030302 biochemistry & molecular biology
Caspase 1
biology.organism_classification
medicine.disease
Molecular biology
Recombinant Proteins
Open reading frame
Cysteine Endopeptidases
Kinetics
Insect Science
Protein Biosynthesis
[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology
DNA
Viral
Rabbits
Leporipoxvirus
Research Article
Zdroj: Journal of Virology
Journal of Virology, American Society for Microbiology, 1996, 70 (9), pp.5860-5866
ISSN: 0022-538X
1098-5514
Popis: A genomic library of myxoma virus (MV) DNA, a leporipoxvirus that causes myxomatosis, was constructed andscreenedbyinvitrotranscription-translation.Aclonewasselectedonthebasisofitsstrongreactivitywith MV antiserum. Analysis of the corresponding DNA sequence and the deduced amino acid sequence revealed an open reading frame coding for a 34-kDa protein with strong homologies to members of the serpin superfamily. The gene encoding this new protein, calledserp2, was localized on the MV genome. Interestingly, thisgeneisdeletedinanattenuatedstrain.WeconstructedabaculovirusvectortoproducerecombinantSerp2 proteinandraisedspecificantiserathatallowedthecharacterizationofSerp2expressionduringtheMVcycle. The biological relevance of this new serpin from MV was monitored, and it was shown that Serp2 could inhibit human interleukin-1b-converting enzyme activity. Myxoma virus (MV) is a leporipoxvirus responsible for myxomatosis, a disease that causes severe losses of the European
Databáze: OpenAIRE
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