Biophysical Investigation and Antitumor Potential of Heterocyclic Palladium-Based Agent: Cytotoxicity, Spectroscopic and Molecular Docking Approaches in Interaction with Human Serum Albumin
| Autor: | Reihaneh Sabbaghzadeh, Maryam Saeidifar, Navid Ahmadi Nasab |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Polymers and Plastics
010405 organic chemistry Chemistry Organic Chemistry chemistry.chemical_element 010402 general chemistry Human serum albumin 01 natural sciences Combinatorial chemistry 0104 chemical sciences Cancer treatment Förster resonance energy transfer Docking (molecular) Materials Chemistry medicine Cytotoxicity medicine.drug Palladium |
| Zdroj: | Polycyclic Aromatic Compounds. 42:91-109 |
| ISSN: | 1563-5333 1040-6638 |
| Popis: | With the aim of cancer treatment, interaction between 1-hexyldithiocarbamato 1,10-phenanthrolinepalladium(II) nitrate complex ([Pd(Hex-Dtc)(Phen)]NO3, Pd-HDP), as a potential antitumor candidate and human serum albumin (HSA), was investigated at four temperatures under physiological conditions. The cell viability of Pd-HDP on normal cell lines, HFFF2 and cancer cell lines, HeLa, and G-292 were also tested. Changes in fluorescence intensity, 3D-fluorescence, synchronous fluorescence, absorption, and circular dichroism spectra of HSA were due to binding with Pd-HDP. The quenching mechanism was suggested as a combination of dynamic and static. The binding constant (K �� 104) and the number of binding sites (n ��� 1) were also determined. Thermodynamic parameters proved that the interaction process was spontaneous (��G H S |
| Databáze: | OpenAIRE |
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