Reversed-phase high-performance liquid chromatographic assay method for quantitating 6-mercaptopurine and its methylated and non-methylated metabolites in a single sample
| Autor: | Yasuhiro Kato, Hideo Mawatari, Shin-Ichiro Nishimura, Kazuhiro Ueda, Nobuo Sakura |
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| Rok vydání: | 1998 |
| Předmět: |
Detection limit
Quality Control Antimetabolites Antineoplastic Chromatography Erythrocytes Mercaptopurine Metabolite Reproducibility of Results General Chemistry Reversed-phase chromatography Precursor Cell Lymphoblastic Leukemia-Lymphoma High-performance liquid chromatography chemistry.chemical_compound Red blood cell Hemoglobins medicine.anatomical_structure chemistry medicine Humans Hemoglobin Thioguanine Quantitative analysis (chemistry) Chromatography High Pressure Liquid medicine.drug |
| Zdroj: | Journal of chromatography. B, Biomedical sciences and applications. 716(1-2) |
| ISSN: | 1387-2273 |
| Popis: | Methods of assaying 6-mercaptopurine (6MP) and its methylated and non-methylated metabolites are essential for the therapeutic dose in treating patients with acute lymphoblastic leukemia. However, previous methods are technically complicated and unsuitable for clinical use. Thus, we have now developed a method utilizing reversed-phase high-performance liquid chromatography (HPLC) in order to quantify these compounds in human red blood cells (RBCs) in a single sample to serve as an index of cytotoxic activity. The agents 6MP, 6-thioguanine (6TG) and 6-methylmercaptopurine (6MMP) were well separated by this assay. Linear relationships were observed between the peak areas and the RBC concentrations of 6MP, 6TG and 6MMP over the range of 20-2000, 18-1800 and 18-1800 pmol per 25 mg hemoglobin (Hb), respectively. The limit of quantitation of the assay is 20, 18 and 18 pmol per 25 mg Hb, respectively. This assay system is suitable for routine clinical use. |
| Databáze: | OpenAIRE |
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