Utilization of cell-transferred cytologic smears in detection of EGFR and KRAS mutation on adenocarcinoma of lung
Autor: | Melissa L. Randolph, Joseph P Eaton, Shaoxiong Chen, Kelly J. Jones, Harvey M. Cramer, Kristin M. Post, Joyashree D. Sen, Amanda J. Malek, Howard H. Wu, Liang Cheng, Sarah A. Bilbo |
---|---|
Rok vydání: | 2013 |
Předmět: |
Adult
Male Pathology medicine.medical_specialty Lung Neoplasms Cell Immunocytochemistry DNA Mutational Analysis Adenocarcinoma medicine.disease_cause Pathology and Forensic Medicine Proto-Oncogene Proteins p21(ras) Cytology Proto-Oncogene Proteins Adenocarcinoma of the lung medicine Humans Lung Aged Aged 80 and over medicine.diagnostic_test business.industry Middle Aged medicine.disease ErbB Receptors Fine-needle aspiration medicine.anatomical_structure ras Proteins Female KRAS business |
Zdroj: | Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc. 27(7) |
ISSN: | 1530-0285 |
Popis: | Cell-transfer technique has been proven useful for performing immunocytochemistry on fine-needle aspiration smears. However, its utility for EGFR and KRAS molecular testing has not been validated. Molecular testing was performed using the cell-transfer technique on both Papanicolaou-stained ethanol-fixed and Hema 3-stained air-dried smears from 32 fine-needle aspiration samples that had diagnoses of adenocarcinoma of the lung, and then was compared to the results of the corresponding formalin-fixed paraffin-embedded tissues. The molecular testing was successfully performed on 32 of 32 ethanol-fixed and 31 of 32 air-dried samples. The molecular results on ethanol-fixed and air-dried smears showed 100% agreement. There is 100% (32/32) agreement for the EGFR and 97% (31/32) agreement for the KRAS between the cell-transfer technique and formalin-fixed paraffin-embedded tissues. One discrepant case was due to low percentage of tumor cells on the smears. Cell-transfer technique is a reliable alternative method for EGFR and KRAS testing if the cell blocks lack adequate cellularity. |
Databáze: | OpenAIRE |
Externí odkaz: |