Tissue-Engineered Cartilage Using an Injectable andin SituGelable Thermoresponsive Gelatin: Fabrication andin VitroPerformance
| Autor: | Shinichi Ibusuki, Yukihide Iwamoto, Takehisa Matsuda, Yasuo Fujii |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Cartilage
Articular In situ Scaffold Hot Temperature Fabrication food.ingredient Materials science Compressive Strength Acrylic Resins Tissue engineered cartilage Gelatin Collagen Type I Injections Biopolymers Chondrocytes food Materials Testing medicine Animals Collagen Type II Cells Cultured Glycosaminoglycans Tissue Engineering Cartilage Cell Cycle General Engineering food and beverages Cell Differentiation In vitro Molecular Weight medicine.anatomical_structure Rabbits Ditiocarb Chondrogenesis Gels Cell Division Biomedical engineering |
| Zdroj: | Tissue Engineering. 9:371-384 |
| ISSN: | 1557-8690 1076-3279 |
| Popis: | An injectable and in situ gelable scaffold can fully fill the space of cartilaginous defects of complex shapes. The authors attempted to develop a novel injection-driven technique for cartilage repair using a thermoresponsive gelatin, poly(N-isopropylacrylamide)-grafted gelatin (PNIPAAm-gelatin). A mixed solution of chondrocytes was isolated from a Japanese white rabbit and PNIPAAm-gelatin was spontaneously solidified at 37 degrees C and cultured. The number of cells in the gel with a poly(N-isopropylacrylamide) (PNIPAAm) chain of high molecular weight (1.3 x 10(5) g/mol) and at low concentration (5 w/v%) remained unchanged irrespective of culture time, and minimal cell death and little cell proliferation were observed. A round-shaped morphology was dominantly restored even at 1 week of incubation. The cell population in the G(0)/G(1) phase was high (more than 90%), and this gradually increased with culture time. Type II collagen and sulfated glycosaminoglycan (s-GAG) were detected in the tissue-engineered cartilage, but a small amount of type I collagen was also detected. Total collagen and s-GAG increased in level close to those of native hyaline cartilage over 12 weeks of culture. Mechanical properties of the tissue-engineered cartilage responding to loading and unloading of compression force tend to approach those of native hyaline cartilage with culture time. These results suggest that PNIPAAm-gelatin may be a suitable in situ formable scaffold for cartilage repair. |
| Databáze: | OpenAIRE |
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